摘要
建立同时检测牛乳基质样品中19种β-受体激动剂类兽药残留的方法。样品经酸化乙腈溶液提取,利用Oasis PRiME HLB固相萃取小柱通过式净化处理除去磷脂,使用超高效液相色谱-串联质谱仪进行检测。结果表明:经过前处理方法与仪器条件优化后,19种β-受体激动剂质量浓度在0~10 ng/mL呈良好线性关系(R2≥0.995);牛乳中19种β-受体激动剂添加量为0.2~1.0μg/kg时,加标回收率均为70%~110%,相对标准偏差<15%。本检测方法前处理简单快捷,具有较高灵敏度和稳定性,可以满足牛乳中β-受体激动剂类兽药残留的快速检测。
This study established a method for the simultaneous detection of 19β-agonist veterinary drug residues in milk samples.The samples were extracted with acidified acetonitrile solution,and the phospholipids were removed by using an Oasis PRiME HLB solid-phase extraction cartridge.The analytes were detected by ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Under optimized pretreatment and instrumental conditions,all analytes showed a good linear relationship in the concentration range of 0 to 10 ng/mL(R2≥0.995).The average recovery rates for milk samples spiked at levels of 0.2 to 1.0μg/kg were between 70%and 110%,and the precision relative standard deviation(RSD)was less than 15%.The method had the advantages of simple and fast pretreatment,and high sensitivity and stability,and therefore could be used for the rapid detection ofβ-agonist veterinary drug residues in milk.
作者
关学农
张敏
苏运聪
王瑶
李飞
李兴佳
杜利卫
张耀广
柴艳兵
GUAN Xuenong;ZHANG Min;SU Yuncong;WANG Yao;LI Fei;LI Xingjia;DU Liwei;ZHANG Yaoguang;CHAI Yanbing(Hebei Food Industry Association,Shijiazhuang 050021,China;Key Laboratory of Dairy Quality and Safety Control,Ministry of Agriculture and Rural Affairs,Junlebao Dairy Co.Ltd.,Shijiazhuang 050021,China)
出处
《乳业科学与技术》
2020年第4期23-28,共6页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
