摘要
目的:许旺细胞能够促进损伤的周围神经再生,可在周围神经组织工程修复中充当种子细胞,其大量高效的培养及纯化是临床治疗周围神经损伤的基础。文章总结近10年许旺细胞体外培养的相关文献,对许旺细胞培养及纯化的研究进展作一描述性综述,以期为许旺细胞的体外培养提供参考。方法:检索有关许旺细胞分离、培养及纯化的文献,以“schwann cells;isolation;culture;purification”为关键词,在PubMed、Web of Science和Medline等数据库进行检索,以“许旺细胞,分离,培养,纯化”为关键词,在CNKI、万方、维普等数据库进行检索。对检索获得的全部文献阅读、分析、判断,最终纳入符合标准的62篇文献进行归纳总结。结果:许旺细胞培养的经典方法为组织块培养法、酶消化法。纯化方法包括抗有丝分裂法、免疫选择法、特异黏附法、预变性、冷喷射、差速贴壁法、层粘连蛋白包被法、低血清法、刺激因子法、荧光激活细胞分选或磁激活细胞分选、免疫抗体包被筛选法、体外冲击波刺激等。结论:许旺细胞体外培养及纯化方法多样,每种方法均有其优缺点,如何快速、高效地得到高纯度许旺细胞仍具挑战,可联合多种方法纯化,尽可能控制许旺细胞增殖的影响因素。
OBJECTIVE:Schwann cells can promote the regeneration of damaged peripheral nerves and serve as seed cells in the engineering repair of peripheral nerve tissue.The effective culture and purification of Schwann cells are the basis of clinical treatment for peripheral nerve injury.This paper summarized the literature of culture of Schwann cells in vitro in recent ten years and made a descriptive review on the research progress of Schwann cell culture and purification,in order to provide references for culture of Schwann cells in vitro.METHODS:Literature related to Schwann cell isolation,culture and purification was retrieved from PubMed,Web of Science,Medline databases,CNKI,Wanfang,VIP and other databases.The keywords included“Schwann cells;isolation;culture;purification”.All the papers obtained from the search were read,analyzed and judged,and finally 62 papers meeting the standards were included.RESULTS:The classical methods of Schwann cell culture include tissue block culture and enzyme digestion.Purification methods include mitosis resistance,immune selection,specific adhesion,pre degeneration,cold jet,differential adherent,laminin package,low serum,stimulating factor,fluorescence activated cell sorting or magnetic activated cell sorting,immunopanning,and extracorporeal shock wave treatment.CONCLUSION:Schwann cells can be cultured and purified in various ways in vitro,and each method has its advantages and disadvantages.How to obtain highpurity Schwann cells quickly and efficiently is still a challenge.Multiple methods can be combined for purification and affective factors can be controlled for Schwann cell proliferation as much as possible.
作者
曾燕华
郝延磊
Zeng Yanhua;Hao Yanlei(College of Clinical Medicine,Jining Medical University,Jining 272000,Shandong Province,China;Department of Neurology,Affiliated Hospital of Jining Medical University,Jining 272000,Shandong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第7期1135-1141,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金项目(81771360),项目负责人:郝延磊。
关键词
干细胞
许旺细胞
组织工程
培养
细胞分选
神经再生
体外冲击波
系统综述
stem cells
Schwann cells
tissue engineering
culture
cell sorting
nerve regeneration
extracorporeal shock wave
systematic review
