摘要
本研究旨在研讨壳寡糖(COS)对DSS诱导溃疡性结肠炎小鼠的保护作用,为进一步开发利用壳寡糖提供依据。本实验通过饮用含3%葡聚糖硫酸钠(DSS)的水7天造模,将50只雄性C57BL/6小鼠随机分为5组:空白组、模型组、阳性组(SASP,50 mg/kg)和壳寡糖低、高剂量组(70和140 mg/kg)。通过体重变化和疾病活动指数、病理学评分、髓过氧化物酶活性评估壳寡糖的作用。通过ELISA检测结肠炎症因子水平。结果显示,壳寡糖干预后能降低DSS诱导小鼠的体重损失、DAI和MPO活性(P>0.05),显著降低结肠组织的IL-6和TNF-α水平(P<0.05),其中壳寡糖低剂量组能极显著改善结肠缩短症状、保护结肠结构(P<0.01),效果优于高剂量组。表明人体推荐剂量的壳寡糖(70 mg/kg)对DSS诱导的结肠炎小鼠具有良好的保护作用,可能与抑制中性粒细胞浸润和炎症有关。
The aim of our study was to investigate the protective role of chitooligosaccharides(COS)on dextran sulfate sodium(DSS)-induced colitis in mice,which might provide a theoretical reference for the development and utilization of COS.In this study,ulcerative colitis was induced by oral administration of 3%DSS for 7 days in drinking water.A total of 50 male C57BL/6 mice were randomly divided into 5 groups:control group,model group,positive control group with sulfasalazine(SASP,50 mg/kg),model group with a low dose of COS(70 mg/kg)and the high dose of COS(140 mg/kg).The protective effect of COS was assessed by weight change and disease activity index(DAI),colon length,histopathological scores and myeloperoxidase(MPO).Inflammation cytokines in colon tissue were detected by ELISA.COS supplement decreased weight loss,DAI and the MPO activities(P>0.05),also significantly reduced the levels of Interleukin(IL)-6,tumor necrosis factor(TNF)-αin the colon tissue,in DSS-treated mice(P<0.05).Additionally,70 mg/kg COS showed a significantly protective effect on colon length and colonic structure in response to DSS challenge(P<0.01),which was better than that of 140 mg/kg COS.In conclusion,COS(70 mg/kg,transferring from a recommended dosage of human)showed obvious protective effects against DSS-induced colitis,which may be associated with suppressed neutrophil infiltration and inflammation.
作者
陈爽
王斌
徐颖
夏文水
CHEN Shuang;WANG Bin;XU Ying;XIA Wen-shui(School of Food Science and Technology,Jiangnan University;Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province,Wuxi 214122,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第8期1295-1301,共7页
Natural Product Research and Development
基金
国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901104)
国家自然科学基金青年基金(31800679)。
关键词
壳寡糖
溃疡性结肠炎
葡聚糖硫酸钠
炎症
chitooligosaccharides
ulcerative colitis
dextran sulfate sodium
inflammation
