摘要
目的了解东南亚十二节段双链RNA病毒(South-East Asian dodeca RNA viruses,Seadornavirus)的分子进化规律及遗传差异。方法基于Seadornavirus衣壳蛋白基因组序列进行同源性、系统进化、理化性质、抗原表位预测以及三级结构和表面电荷分布的分析。结果Seadornavirus的最近共同祖先大约于2359年前,分为3个进化簇,其进化时间分别为约距今1338、499、253年前,其平均核苷酸替换率为3.6×10-4n/s/y。Seadornavirus 3种病毒之间的核苷酸与氨基酸同源性分别是31.3%~100%(μ=68.0%)和11.9%~100%(μ=56.0%)。理化性质和抗原表位分析显示,版纳病毒(Banna virus,BAV)衣壳蛋白为酸性疏水性蛋白质,存在6个B细胞表位和2个Th抗原表位,而辽宁病毒(Liaoning virus,LNV)和卡迪皮诺病毒(Kadipiro virus,KDV)为碱性亲水性蛋白质,分别存在3和5个B细胞抗原表位,其中LNV仅有1个Th抗原表位,KDV病毒则不存在。三级结构以及蛋白表面电荷分析显示,Seadornavirus的α螺旋和β折叠各不相同,并且BAV有2个明显的带正电荷区域和2个带负电荷的区域,LNV只有1个带正电荷区域,KDV则有2个带正电荷区域。结论Seadornavirus进化速率快,并且其基因组、衣壳蛋白抗原表位、理化性质、三级结构等具有较大的差异。
Objective To understand the molecular evolutionary and genetic differences of South-East Asian dodeca RNA viruses(Seadornavirus).Methods The homology,phylogeny,physicochemical properties,epitope prediction,tertiary structure and surface charge distribution were analyzed based on genome sequence of Seadornavirus capsid proteins.Results The time of most recent common ancestor(tMRCA)of Seadornavirus was estimated to be about 2359 years ago,which were divided into three clusters.Their tMRCA were about 1338,499 and 253 years ago,respectively.And their mean substitution rate of Seadornavirus was 3.6×10-4 n/s/y.The homology of nucleotide and amino acid was 31.3%-100%(μ=68.0%)and 11.9%-100%(μ=56.0%)respectively.Physicochemical properties and epitope analysis showed that the Banna virus(BAV)capsid protein is an acidic hydrophobic protein that there are 6 B cell epitopes and 2 Th epitopes,while Liaoning virus(LNV)and Kadipiro virus(KDV)are alkaline hydrophilic protein that there are 3 and 5 B cell epitopes,respectively.And there is only 1 Th epitope in the capsid protein of LNV,while KDV does not contain this epitope.The tertiary structure and protein surface charge analysis showed that the alpha helices and beta folds of Seadornavirus were different and the BAV contains two distinctly positively charged regions and two negatively charged regions,LNV has only one positively charged region,and KDV has two positively charged regions.Conclusions Seadornaviruses had more rapid evolution and strong adaptability,and the genome sequences,protein epitopes,physicochemical properties and the tertiary structures between Seadornaviruses are different.
作者
张珺
赵秋霞
王家辉
夏菡
王纪衡
张建明
庄莹
刘红
丁忠峰
Zhang Jun;Zhao Qiuxia;Wang Jiahui;Xia Han;Wang Jiheng;Zhang Jianming;Zhuang Ying;Liu Hong;Ding Zhongfeng(The College of Life Sciences,Shandong University of Technology,Zibo 255000,China;The Department of Pediatrics,Yuncheng Central Hospital of Shanxi Province,Yuncheng 044000,China;Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2020年第3期256-263,共8页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金(81301479)
传染病预防控制国家重点实验室自主研究开放课题(2018SKLID309)
湖北省健康委员会科研项目(WJ2019Q060)。
