摘要
目的观察基因组DNA甲基化介导的细胞凋亡在急诊PCI术后急性室壁瘤(VA)形成中的作用。方法选择急性ST段抬高型心肌梗死(STEMI)行急诊PCI治疗的96例患者为病例组,其中包括室壁瘤组55例,非室壁瘤组41例,另选择40例健康体检者为对照组。采用ELISA法测定基因组DNA甲基化水平,应用RT-PCR法测定bad mRNA水平。比较各组的DNA甲基化及bad mRNA水平,并分析相关性。结果病例组DNA甲基化水平低于对照组,bad mRNA水平高于对照组(P<0.05)。室壁瘤组DNA甲基化水平低于非室壁瘤组,bad mRNA水平高于非室壁瘤组(P<0.05)。随着基因组DNA甲基化水平的降低,bad mRNA水平增高,二者呈负相关(P=0.012)。结论基因组DNA甲基化介导的细胞凋亡增加了心肌梗死后VA形成的易感性。
Objective To observe the role of genomic DNA methylation mediated apoptosis in acute ventricular aneurysm(VA)formation after emergency PCI.Methods Ninety-six acute ST segment elevation myocardial infarction(STEMI)patients treated with emergency PCI were selected as case group,including 55 patients of ventricular aneurysm group,41 patients of non-ventricular aneurysm group.Another 40 cases of healthy examination persons were selected as control group.The level of DNA methylation was measured by ELISA method,and bad mRNA level was measured by RT-PCR method.The levels of DNA methylation and bad mRNA in each group were compared,and the correlation was analyzed.Results The level of DNA methylation in the case group was lower than that in the control group,and bad mRNA level was higher than that in the control group(P<0.05).The level of DNA methylation in the ventricular aneurysm group was lower than that in the non-ventricular aneurysm group,and bad mRNA level was higher than that in the non-ventricular aneurysm group(P<0.05).With the decrease of DNA methylation level,bad mRNA level increased,and there was a negative correlation between them(P=0.012).Conclusion Genomic DNA methylation mediated apoptosis increases the susceptibility of VA formation after myocardial infarction.
作者
姚宗芹
张金祝
李兴帼
付存玉
王红霞
YAO Zongqin;ZHANG Jinzhu;LI Xingguo;FU Cunyu;WANG Hongxia(Cardiovascular Medicine Department,Linyi Central Hospital,Linyi 276400;Internal Medicine Department,Linyi Communications Hospital,Linyi 276000,China)
出处
《临床医学研究与实践》
2020年第23期120-121,共2页
Clinical Research and Practice
关键词
急性ST段抬高型心肌梗死
急诊PCI
DNA甲基化
室壁瘤
acute ST segment elevation myocardial infarction
emergency PCI
DNA methylation
ventricular aneurysm
