摘要
目的分析慢性应激诱导脂肪二肽基肽酶-4(DPP-4)表达及对脂肪炎症和糖代谢的影响。方法雄性SPF级小鼠20只随机分慢性应激(Stress)组和正常对照(Control)组。Stress组小鼠每天在自制式束缚器中限制活动2 h,实验持续14 d。采用免疫组化方法检测巨噬细胞表面标志物(CD11b)在脂肪中的表达,实时定量PCR法检测脂肪组织中DPP-4、细胞因子(Adiponectin、MCP-1、IL-6、TNF-α)及糖代谢(IRS-1、GLUT-4)等指标的mRNA的相对表达量;ELISA法检测DPP-4酶活性及GLP-1浓度。结果Stress小鼠腹部白色脂肪组织(WAT)与Control组相比显著的退缩及其重量显著降低(P<0.001)。Stress组WAT出现大量的单核细胞、中性粒细胞浸润反应和炎症性改变;Stress显著降低Adiponectin的表达,并显著增高MCP-1、IL-6、TNF-α的mRNA表达及其血液中的浓度(P<0.001);Stress组WAT组织中DPP-4 mRNA表达及其血液中的活性显著增高,而GLP-1血液中的浓度显著降低(P<0.001);Stress组WAT组织中IRS-1及GLUT-4的mRNA水平显著低于Control组(P<0.001)。结论慢性应激诱导脂肪DPP-4异常表达,进而引起脂肪炎症和糖代谢异常等反应。
Objective To analyze chronic stress induced dipeptidyl peptidase-4(DPP-4)expression and its role in adipose inflammation and glycometabolism.Methods 20 male SPF mice were randomly divided into two groups:a stress group and a control group.Mice in the stress group were restrained in our own restraint device for 2 hours per day and lasted for 14 days.The expression of macrophage surface marker(CD11 b)in adipose tissue was detected by immunohistochemistry.The expression of DPP-4,adipocytokines(Adiponectin,MCP-1,IL-6,TNF-α)in adipose tissue and the expressions of glucose metabolism molecules(IRS-1,GLUT-4)were further detected by RT-PCR.The enzymatic activity of DPP-4 and the concentration of GLP-1 were detected by ELISA.Results The white adipose tissue(WAT)of the stress group mice significantly retracted and WAT weight significantly reduced(P<0.001).WAT in the stress group showed a large number of monocytes,neutrophils infiltration and inflammatory changes.Mice in the stress group significantly decreased the expression of Adiponectin and significantly increased the expression of MCP-1,IL-6 and TNF-α(P<0.001).DPP-4 expression in WAT tissues and its activity in blood increased significantly in the stress group,but the concentration of serum GLP-1 decreased significantly(P<0.001).The mRNA level of IRS-1 and mRNA level of GLUT-4 in WAT of the stress group were significantly lower than that of the control group(P<0.001).Conclusions Chronic stress induced abnormal expression of DPP-4 in adipose tissue,which can lead to adipose inflammation and abnormal glycometabolism.
作者
阿不来克木·马合木提
买买提·依斯热依力
艾克拜尔·艾力
李义亮
阿孜古丽·阿力木江
闫晶
克力木·阿不都热依木
ABULAIKEMU-Mahemuti;MAIMAIT-Tisireyili;AIKEBAIER-Aili;LI Ti-liang;AZIGULI-Alimujiang;YAN Jing;KELIMU-Abudureyimu(Department of Emergency and Traumatic Surgery,,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China;Research Institute of General and Minimally Invasive Surgery,,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China;Department of Minimally Invasive Surgery,Hernia and Abdominal Wall Surgery,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China)
出处
《中国临床解剖学杂志》
CSCD
北大核心
2020年第4期438-443,共6页
Chinese Journal of Clinical Anatomy
基金
新疆维吾尔自治区自然科学基金(2018D01C134)
新疆维吾尔自治区引进高层次人才天池百人计划项目(201939)。
关键词
慢性应激
二肽基肽酶-4
脂肪细胞因子
炎症
糖代谢
Chronic stress
Dipeptidyl peptidase-4
Adipocytokine
Inflammation
Glycometabolism
