摘要
目的初步探索中国人群中唾液1,5-脱水葡萄糖醇(1,5-AG)的正常参考范围,为临床推广应用这一无创检测手段提供依据。方法2018年9月至2019年6月于上海交通大学附属第六人民医院招募224例正常糖代谢受试者(20~69岁,男59例、女165例),通过1 min咀嚼40~50次Salivette唾液采集管收集唾液,采用液相色谱质谱联用(LC-MS)检测唾液1,5-AG水平,采用酶法测定血清1,5-AG水平。组间比较采用独立样本t检验、方差分析、Wilcoxon秩和检验及Kruskal-Wallis检验。采用Spearman相关分析及多元线性回归研究唾液1,5-AG与其他指标的关系。结果(1)224例正常人中唾液1,5-AG平均水平为0.53(0.35~0.77)mg/L,且无性别差异(P=0.118)。(2)将研究对象按照年龄分为20~29岁、30~49岁、50~69岁三个亚组,无论男性或女性,唾液1,5-AG水平在3个年龄亚组间差异无统计学意义(均P>0.05)。(3)根据体质指数(BMI)水平将研究对象分为18.5~20.9、21.0~22.9和23.0~24.9 kg/m2三个亚组,无论男性或女性,唾液1,5-AG在3个BMI亚组间差异均无统计学意义(均P>0.05)。(4)唾液1,5-AG的正常参考范围(2.5%~97.5%)为0.09~1.63 mg/L,血清1,5-AG是唾液1,5-AG的唯一独立影响因素(标准化β=0.279,t=3.636,P<0.01)。结论基于LC-MS检测方法,唾液1,5-AG的正常参考范围为0.09~1.63 mg/L。唾液1,5-AG不受性别、年龄及BMI影响,临床应用无创便捷且较为稳定,可能成为糖尿病患者血糖监测的另一补充工具。
Objective To explore the normal reference interval of saliva 1,5-anhydroglucitol(1,5-AG)in Chinese population,and to give evidence for clinical application of the noninvasive examination method.Methods A total of 224 subjects with normal glucose tolerance(aged 20-69,59 male and 165 female)were enrolled in Shanghai Jiao Tong University Affiliated Sixth People′s Hospital from September 2018 to June 2019 and chewed the cottons in the Salivette tubes 40-50 times for 1 minute to collect saliva.Saliva 1,5-AG levels were measured using liquid chromatography-mass spectrometry(LC-MS)and serum 1,5-AG levels were measured with enzymatic method.The independent sample t test,analysis of variance,Wilcoxon rank sum test and Kruskal-Wallis test were used for the inter-group comparison.Spearman correlation analysis and multiple linear regression were used to study the relationship between saliva 1,5-AG and other indicators.Results(1)The average level of saliva 1,5-AG in 224 normal subjects was 0.53(0.35-0.77)mg/L,and there was no gender difference in saliva 1,5-AG levels(P=0.118).(2)According to age the subjects were divided into three subgroups:20-29 years,30-49 years and 50-69 years.There were no significant differences in saliva 1,5-AG levels among three age subgroups in both male and female(all P>0.05).(3)According to body mass index(BMI)the subjects were divided into three subgroups:18.5-20.9 kg/m2,21.0-22.9 kg/m2 and 23.0-24.9 kg/m2.There were no significant differences in saliva 1,5-AG levels among three BMI subgroups in both male and female(all P>0.05).(4)The normal reference interval of saliva 1,5-AG(the 2.5-97.5 percentiles)was 0.09-1.63 mg/L,serum 1,5-AG was the only independent factor associated with saliva 1,5-AG(standardizedβ=0.279,t=3.636,P<0.01).Conclusion Based on LC-MS determination method the normal reference interval of saliva 1,5-AG was 0.09-1.63 mg/L.Saliva 1,5-AG is not affected by gender,age and BMI and is noninvasive,convenient and robust for clinical application and may become another complementary tool for blood glucose monitoring in diabetes.
作者
菅朝慧
赵爱华
马晓静
陆蔚
朱玮
王煜非
周健
包玉倩
Jian Chaohui;Zhao Aihua;Ma Xiaojing;Lu Wei;Zhu Wei;Wang Yufei;Zhou Jian;Bao Yuqian(Department of Endocrinology and Metabolism,Shanghai Jiao Tong University Affiliated Sixth People′s Hospital,Shanghai Clinical Center for Diabetes,Shanghai 200233,China;Center for Translational Medicine,Shanghai Jiao Tong University Affiliated Sixth People′s Hospital,Shanghai 200233,China)
出处
《中华糖尿病杂志》
CAS
CSCD
北大核心
2020年第7期480-485,共6页
CHINESE JOURNAL OF DIABETES MELLITUS
基金
市级医院新兴前沿技术联合攻关项目(SHDC12016108)
上海市教育委员会高峰高原学科建设计划(20161430)。
关键词
血糖监测
唾液检测
1
5-脱水葡萄糖醇
液相色谱质谱联用
正常参考范围
Glucose monitoring
Saliva determination
1,5-Anhydroglucitol
Liquid chromatography-mass spectrometry
Normal reference interval
