摘要
目的:观察瓜蒌薤白半夏汤对心肌梗死后大鼠心功能及心肌纤维化的影响。方法:将36只雄性SD大鼠随机分为空白组、假手术组和手术组;空白组和假手术组每组各6只;手术组采用冠状动脉左前降支结扎联合高脂饮食法复制大鼠心肌梗死痰浊壅盛模型,将造模成功的大鼠随机分为模型组、瓜蒌薤白半夏汤组(GXBT)和雅施达组,假手术组只穿线不结扎。空白组、假手术组和模型组给予10 mL·kg^-1·d^-1的生理盐水灌胃,GXBT组2. 68 g·kg^-1·d^-1水煎液灌胃,雅施达组0. 36 mg·kg^-1·d^-1灌胃。造模4周后行心脏超声检测心功能以验证模型成功;8周后再次行心脏超声检测大鼠心梗后心功能变化,之后处死大鼠取材;苏木素-伊红(HE)染色观察大鼠心肌组织的病理变化;Masson染色观察大鼠心肌纤维化程度;酶联免疫吸附测定(ELISA)检测大鼠血清中B型钠尿肽(BNP)和半乳糖凝集素-3(Gal-3)的变化;蛋白免疫印迹法(Western blot)检测大鼠心肌中Gal-3,Ⅰ型胶原(Col-Ⅰ)和Ⅲ型胶原(Col-Ⅲ)蛋白表达的变化。结果:与空白组和假手术组比较,模型组大鼠的左室射血分数(EF)和短轴缩短率(FS)显著减小(P<0. 01),心肌组织有炎性细胞浸润,心肌胶原纤维增多,血清中BNP和Gal-3的含量明显升高(P<0. 05),心肌中Gal-3,Col-Ⅰ和Col-Ⅲ的蛋白表达显著增多(P<0. 01);与模型组比较,GXBT组EF值和FS值明显升高(P<0. 05),心肌细胞形态结构改善,胶原纤维减少,血清中BNP和Gal-3的蛋白表达明显降低(P<0. 05),心肌组织中Gal-3,Col-Ⅰ和Col-Ⅲ的含量明显减少(P<0. 05)。结论:瓜蒌薤白半夏汤能够改善心肌梗死后大鼠的心功能,减轻心肌纤维化,减缓心肌梗死后心力衰竭的进程,其作用机制可能与降低Gal-3的表达有关。
Objective:To observe the effect of Gualou Xiebai Banxiatang(GXBT)on cardiac function and myocardial fibrosis in rats after myocardial infarction. Method:The 36 male SD rats were randomly divided into blank group,sham group and surgical group,and 6 males in blank group and sham operation group. The model of phlegm obstruction in myocardial infarction of rats was replicated by ligation of left anterior descending coronary artery and high fat diet,and the successful rats were randomly divided into 3 groups:model group,GXBT group and acertil group. In the sham group,only the threading was not ligated. The blank group and the sham group and the model group were given 10 mL·kg^-1·d^-1 of normal saline,and 2. 68 g·kg^-1·d^-1 of the GXBT group were given intragastric administration,and 0. 36 mg·kg^-1·d^-1 was given intragastrically in acertil group.After 4 weeks of model,the heart function was detected by heart ultrasound to verify the success of the model.After 8 weeks,the heart function of the heart of the rat was detected by heart ultrasound again,and then the samples were sacrificed. The pathological changes of the myocardial cells of the rats were observed with hematoxylin-eosin(HE)staining,and the degree of myocardial fibrosis in the rats was observed by Masson staining. The changes of serum B-type natriuretic peptide(BNP)and galectin-3(Gal-3)in rat serum were detected by enzyme-linked immunosorbent assay(ELISA)method,and the expression of Gal-3,Collagen Ⅰ(Col-Ⅰ )and Collagen Ⅲ(Col-Ⅲ)was detected by Western blot. Result: Compared with blank group and sham group,the left ventricular ejection fraction(EF)and short-axis shortening rate(FS)of model group were significantly decreased(P<0. 01),the infiltration of inflammatory cells,the increase of the myocardial collagen fibers,the contents of BNP and Gal-3 in the serum were increased(P<0. 05). The expression of Gal-3,Col-Ⅰand Col-Ⅲ in the myocardial tissue increased significantly(P<0. 01). Compared with the model group,EF and FS of GXPD were significantly increased(P<0. 05),the morphological structure of myocardial cells was improved,the collagen fiber was decreased. The expression of BNP and Gal-3 in serum decreased significantly(P<0. 05),and the content of Gal-3,Col-Ⅰ and Col-Ⅲ in myocardial tissue was decreased(P<0. 05).Conclusion: Gualou Xiebai Banxiatang can improve cardiac function,reduce myocardial fibrosis and slow down the process of heart failure after myocardial infarction in rats with myocardial infarction. Its mechanism may be related to the decrease of Gal-3 expression.
作者
刘彩红
李洪雷
张倩
田升
张立娟
王媛
王世军
LIU Cai-hong;LI Hong-lei;ZHANG Qian;TIAN Sheng;ZHANG Li-juan;WANG Yuan;WANG Shi-jun(Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第16期50-55,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山东省重点产业关键技术项目(2016CYJS08A01-3,2016CYJS08A01-4)
山东省自然科学基金重大基础研究项目(ZR2019ZD23)。
