摘要
Pigs are one of the most important domesticated animals and have great value in agriculture and biomedicine.Single nucleotide polymorphisms(SNPs)are a dominant type of genetic variation among individual pigs and contribute to the formation of traits.Precision single base substitution provides a strategy for accurate genetic improvement in pig production with the characterization of functional SNPs and genetic variants in pigs.Base editing has recently been developed as the latest gene-editing tool that can directly make changes in single nucleotides without introducing double-stranded DNA breaks(DSBs),providing a promising solution for precise genetic modification in large animals.This review summarizes gene-editing developments and highlights recent genetic dissection related to SNPs in major economic traits which may have the potential to be modified using SNP-editing applications.In addition,limitations and future directions of base editing in pig breeding are discussed.
基金
supported by the National Natural Science Foundation of China(81671274,31925036,31272440,and 31801031)
the National Transgenic Project of China(2016ZX08009003006-007)
the Elite Youth Program of the Chinese Academy of Agricultural Sciences(ASTIP-IAS05)。
