摘要
目的探究血清高迁移率族蛋白B1(HMGB1)、脑钠尿肽(BNP)水平与新生儿持续性肺动脉高压(PPHN)的关系。方法选取2016年12月—2019年10月保定市第一中心医院收治的46例PPHN患儿作为PPHN组,选取同期住院的46例非持续性肺动脉高压新生儿作为对照组。采用酶联免疫吸附法(ELISA)检测血清HMGB1、BNP、肿瘤坏死因子-α(TNF-α)、内皮素-1(ET-1)水平,以Pearson法分析PPHN患儿血清HMGB1、BNP水平与TNF-α、ET-1的相关性,采用受试者工作特征曲线(ROC)评价血清HMGB1、BNP对PPHN的诊断价值。结果PPHN组患儿血清HMGB1、BNP、TNF-α、ET-1水平均明显高于对照组(P均<0.05);PPHN患儿血清HMGB1、BNP水平与TNF-α、ET-1均呈正相关(P均<0.05);血清HMGB1、BNP对PPHN诊断的曲线下面积(AUC)为0.840,0.796,截断值分别为7.62 ng/mL、0.63 ng/mL,相应灵敏度分别为76.1%,73.9%,特异度分别为84.8%,87.0%;两者联合诊断PPHN的AUC为0.895,其灵敏度、特异度分别为89.1%,87.0%。结论HMGB1、BNP在PPHN患儿血清中均显著升高,两者可能与TNF-α、ET-1协同促进PPHN发展,两者联合检测可有效提高对PPHN的诊断价值,有利于筛查PPHN患儿。
Objective It is to investigate the relationships between serum high mobility group protein box 1(HMGB1),brain natriuretic peptide(BNP)and persistent pulmonary hypertension of newborn(PPHN).Methods A total of 46 children with PPHN admitted to the First Central Hospital of Baoding from December 2016 to January 2020 were selected for the study,named as PPHN group;another 46 children with non persistent pulmonary hypertension in the same period were selected as the control group.The levels of serum HMGB1,BNP,tumor necrosis factor-α(TNF-α)and endothelin-1(ET-1)were measured by enzyme-linked immunosorbent assay(ELISA);Pearson method was used to analyze the correlation between serum HMGB1,BNP levels and TNF-α,ET-1 in PPHN children;and the diagnostic value of serum HMGB1 and BNP in PPHN was evaluated by receiver operating characteristic curve(ROC).Results The levels of serum HMGB1,BNP,TNF-αand ET-1 in PPHN group were significantly higher than those in the control group(P<0.05);the levels of serum HMGB1 and BNP of PPHN children were positively correlated with TNF-αand ET-1(P<0.05);the area under curve(AUC)of serum HMGB1 and BNP in diagnosis of PPHN was 0.840 and 0.796,the cutoff value was 7.62 ng/mL and 0.63 ng/mL respectively,the corresponding sensitivity was 76.1%and 73.9%,and the specificity was 84.8%and 87.0%,respectively;meanwhile,the AUC of the combination in the diagnosis of PPHN was 0.895,and the sensitivity and specificity were 89.1%and 87.0%,respectively.Conclusion HMGB1 and BNP are significantly increased in PPHN children’s serum,which may act with TNF-αand ET-1,and then affect the development of PPHN.The combination of HMGB1 and BNP can effectively improve the diagnostic value of PPHN and help to screen PPHN children.
作者
高宁
杨梅
吴娜
GAO Ning;YANG Mei;WU Na(The First Central Hospital of Baoding, Baoding 071000, Hebei, China)
出处
《现代中西医结合杂志》
CAS
2020年第23期2551-2555,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
保定市科技计划项目(1941ZF056)。
关键词
新生儿持续性肺动脉高压
高迁移率族蛋白B1
脑钠尿肽
persistent pulmonary hypertension of newborn
high mobility group protein box 1
brain natriuretic peptide
diagnostic value
