摘要
提出了一种新的实验方法,将阿司匹林乙醇溶液原位浸渍到氧化石墨烯(GO)纳米片中,并通过超临界CO2技术来增强阿司匹林的药物负载和释放性能。该项实验能够评估控制阿司匹林负载和释放过程的关键因素,在相同的操作条件下比较了常规的物理方法与这种超临界CO2技术(SCOT)。紫外光谱数据表明,与物理法负载阿司匹林(0.0363 mg·mg-1)相比,SCOT最佳装载量(0.1210 mg·mg-1)增加了三倍。由于药物均匀分散在GO底物中,使用SCOT技术的药物释放度从物理方法的51.2%提高到95.7%。超临界CO2条件促进了阿司匹林的装载,这归因于其高分散性,零表面张力和消失的气液界面。红外光谱数据表明,GO平面内的含氧基团与阿司匹林分子中羧基的氢原子主要以氢键(1~10 Kcal·mol-1)形成存在,SCOT增强了二者之间的范德华力作用,这种相对弱的非键结合力(0.1~1 Kcal·mol-1)明显促进了药物负载和释放能力。这种采用SC-CO2改善GO负荷阿司匹林的新方法将为生物医学领域提供一个新的平台。
A novel experimental approach was developed to enhance aspirin drug loading and release capacity, by an in-situ impregnation of aspirin ethanol solution into graphene oxide(GO) nanosheets, subjected to supercritical CO2 extraction. This experimental set-up allowed us to evaluate key factors governing loading and release processes of aspirin. A conventional physical method was also carried out to compare with this supercritical CO2 technology(SCOT), under the same operational conditions. Research data obtained from ultraviolet spectroscopy indicated that this newly developed SCOT quadrupled optimal aspirin loading capacity(0.121 0 mg·mg-1), compared with physical approach datum(0.036 3 mg·mg-1). The release capability was improved from 51.2% obtained from physical method to 95.7 % using SCOT due to homogeneous dispersion of drug into GO substrate. The kinetics of aspirin impregnation was facilitated under supercritical conditions, attributed to its high dispersibility, zero surface tension and non-distinguishable gas-liquid interface. The infrared spectroscopic evaluation on bond vibrations indicated the hydrogen bond(1~10 kcal·mol-1) was mainly formed between oxygen-containing groups in GO interplane and hydrogen atoms from the carboxylic groups in aspirin molecules. This van der Waals intermolecular forces enhanced by SCOT significantly facilitated loading and release capability due to its relative weak non-bonding forces(0.1~1 kcal·mol-1). This invented approach employing SC-CO2 to improve GO loading aspirin will provide a new platform in the biomedical fields.
作者
张杰
刘政
张慧明
周淑晶
祝芳芳
解词
张向宇
王莹
ZHANG Jie;LIU Zheng;ZHANG Huiming;ZHOU Shujing;ZHU Fangfang;XIE Ci;ZHANG Xiangyu;WANG Ying(School of Pharmacy,Jiamusi University,Jiamusi 154007,China;School of Basic Medicine,Jiamusi University,Jiamusi 154007,China;Jiamusi Food and Drug Inspection and Testing Center,Jiamusi 154007,China)
出处
《黑龙江大学自然科学学报》
CAS
2020年第3期323-332,共10页
Journal of Natural Science of Heilongjiang University
基金
Supported by the National Natural Science Foundation of China (81601616)
the Excellent Innovation Team Based on the Basic Scientific Research Vocational Cost for the Provincial Undergraduate Universities in Heilongjiang (2018-XYYWF-0914)。
