摘要
为了分离利用高效植物病害生物防治菌株,从广东药用植物金丝皇菊根部分离获得一株木霉菌株Tk1。经过形态观察、r DNA-ITS和EF-1α序列分析明确其为拟康宁木霉Trichodermakoningiopsis。用平板对峙培养法测定,该菌株对金丝皇菊枯萎病菌(尖孢镰刀菌Fusarium oxysporum)和柑橘炭疽病菌(胶胞炭疽菌Colletotrichumgloeosporioides)的抑制率分别为68.5%和77.1%,且在后期能完全覆盖或部分覆盖病原真菌。其无菌发酵液对金丝皇菊枯萎病菌的抑制率为45.55%。该菌株营养生长和产孢的最适碳源分别为乳糖和葡萄糖,最适氮源是酵母膏;在p H 4~11的培养基上均可生长、产孢,最适p H为7;12 h交替光照条件下菌丝的生长速度最快,全光照条件下产孢数量最大;该菌株的致死温度为73℃处理10 min。
A Trichoderma strain of Tk1 was isolated from the root of a medicinal plant Chrysanthemum morifolium"Huangju"in Guangdong.The Tk1 strain was identified to be Trichoderma koningiopsis through morphological observation,rDNA-ITS and EF-1αsequence analysis.The inhibition rates of T.koningiopsis Tk1 against Fusarium oxysporum and Colletotrichum gloeosporioides in dual culture were 68.5%and 77.1%on PDA plates,respectively,and they could completely or partially cover the colonies of the F.oxysporum and C.gloeosporioides.Thefermentation filtrate showed an inhibition rate of 45.55%to the growth of F.oxysporum.Biological characteristics analysis showed that the optimal carbon source for the growth and conidiation was lactose and glucose,while the optimum nitrogen source was yeast extract.The isolate could grow and sporualte on PDA medium with pH 4―11,and the optimal pH value was 7.Under 12 h light and 12 h dark alteration,the growth rate of the mycelium was the fastest while 24 h consecutive light was the most favorable to its conidiation.The lethal temperature of the strain was 73℃for 10 min.
作者
罗梅
罗玉霖
陈沫冰
舒永馨
陈欣瑜
董章勇
LUO Mei;LUO Yulin;CHEN Mobing;SHU Yongxin;CHEN Xinyu;DONG Zhangyong(College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Innovative Institute for Plant Health,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)
出处
《中国生物防治学报》
CSCD
北大核心
2020年第4期581-586,共6页
Chinese Journal of Biological Control
基金
广东省科技创新战略专项(2018B020205003)
广东省普通高校特色创新类项目(2018KTSCX097)
广东省科技特派员项目。
关键词
木霉
生物防治
分离
鉴定
拮抗作用
生物学特性
Trichoderma
biocontrol
isolation
identification
antagonistic effect
biological characteristics
