摘要
探讨等花柱资源在甜荞资源创新中有效利用的方法,为今后甜荞资源创新及新品种选育提供更好的技术支撑。利用甜荞等花柱资源与栽培种不同花型进行正反交杂交试验,在此基础上对等花柱花器×栽培种花器、等花柱花器×长花柱短雄蕊花器、等花柱花器×短花柱长雄蕊花器、栽培种花器×等花柱花器、长花柱短雄蕊花器×等花柱花器、短花柱长雄蕊花器×等花柱花器6个杂交组合后代结籽状况及遗传分离进行观察和分析。结果表明,等花柱甜荞资源本身结实率达22.6%,明显高于普通甜荞,对于等花柱新纯系的筛选以长花柱短雄蕊花器×等花柱花器杂交模式最佳,新品系筛选是等花柱花器×栽培种花器最佳。本研究结果说明,根据资源创新的目的不同,利用等花柱资源采用不同的杂交技术是可行的,后代都产生了明显的遗传变异,能有效解决甜荞杂交育种的瓶颈问题。
In order to provide better guarantee on technical support for the future innovation of common buckwheat resources and new variety breeding,this study investigated the effective method of isostyle resources in innovation of common buckwheat.The positive and negative hybridization tests by using isostyle germplasm resource crossing with common buckwheat cultivars showing different flower types were carried out.Six hybridization combinations(isostyle×cultivar,isostyle×long style,isostyle×short style,cultivar×isostyle,long style×isostyle,short style×isostyle)were achieved,and they were subjected for analyzing the seed-fertility and the inheritance segregation.The results showed that the fertility rate of isostyle common buckwheat resources was observed up to 22.6%,significantly higher than that of common buckwheat.Regarding to the hybrids for selecting the new isostyle pure lines,the"long style×isostyle"hybrid pattern was the best pattern.Otherwise,the"isostyle×cultivar"was optimal for the selection of new strains.Taken together,this study suggested the feasibility on hybridization techniques in order to meet the demand of the resource innovation.The offspring plants were observed with obvious phenotypic variation,which might suggest a possibility to effectively solve the bottleneck problem in common buckwheat cross breeding.
作者
陈稳良
李秀莲
史兴海
梁改梅
刘龙龙
赵建栋
秦秀珍
CHEN Wen-liang;LI Xiu-lian;SHI Xing-hai;LIANG Gai-mei;LIU long-long;ZHAO Jian-dong;QIN Xiu-zhen(School of Agronomy,Shanxi Agricultural University,Taiyuan 030031;Shanxi Institute of Organic Dry-farming Agriculture,Shanxi Agricultural University,Taiyuan 030031;Center for Agricultural Genetic Resources Research,Shanxi Agricultural University,Taiyuan 030031)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第4期1030-1035,共6页
Journal of Plant Genetic Resources
基金
国家燕麦荞麦现代农业产业技术体系专项资金(CARS-07-A-2)
山西省科技攻关项目(201903D221096)
山西省科技成果转化项目(201804D131053,201904D131059)
山西省农业科学院特色农业技术攻关项目(YGG17128)
山西省农业科学院育种工程项目(17yzgc092)
山西省杂粮产业技术体系(sxzljstx-1)。
关键词
甜荞
等花柱资源
杂交
资源创新
common buckwheat
isostyle resource
hybridization
resource innovation
