摘要
浮萍为浮萍科水生植物,具有生长繁殖快、氮磷吸收能力强及高含蛋白质和淀粉的特性,在饲料和能源方面具有较大的开发潜力。目前尚未有系统研究我国长江中游地区浮萍种质资源的报道。本研究从我国长江中游地区3个省42个具有代表性的自然水体中共收集到浮萍种质112份。经形态学并结合分子生物学鉴定,它们分属4个属6个种,其中青萍属(Lemna L.)含3个种54份种质;多根紫萍属(Spirodela Schleid.)、少根紫萍属(Landoltia Les&Crawford)、芜萍属(Wolffia Horkel ex Schleid.)均只有1个种,种质数量分别为33份、19份和6份。遗传多样性分析表明Atp F-Atp H间隔序列和rp S16内含子序列的单倍型数量分别为11和18,单倍型多样性分别为0.748和0.892,群体突变率分别为0.03013和0.03507,平均每Kb核苷酸差异数为31.386和61.263,核苷酸多样性分别为0.04713和0.06623。本研究为我国长江中游浮萍的资源化利用奠定了基础。
Duckweed,a family of aquatic plants,has the characteristics of fast growth and reproduction,strong nitrogen and phosphorus absorption capacity,and high protein and starch content.Therefore it has great potential in feed and bioenergy production.However,the information of duckweed germplasm collection and diversity analysis in the middle reaches of Changjiang River has not been systematically reported.In this study,112 duckweed germplasms were collected from 42 representative natural aquatic habitats in three provinces of the middle reaches of the Changjiang River.These germplasm resources were classified into 4 genera and 6 species by morphological and molecular biological identification.Lemna contained 3 species and 54 accessions.Spirodela,Landoltia and Wolffia contained only 1 species,and their collected germplasm number was 33,19 and 6,respectively.Genetic diversity analysis showed that the number of haplotypes of their AtpF-AtpH spacer sequences and rpS16 intron sequences were 11 and 18,the haplotype diversity was 0.748 and 0.892,the population mutation rates were 0.03013 and 0.03507,nucleotide differences per Kb were 31.386 and 61.263 and nucleotide diversity was 0.04713 and 0.06623,respectively.This study plays a key role for the utilization of duckweed in the middle reaches of the Changjiang River.
作者
穆瑶佳
谭德冠
于博涵
付莉莉
陈婷
孙雪飘
张家明
MU Yao-jia;TAN De-guan;YU Bo-han;FU Li-li;CHEN Ting;SUN Xue-piao;ZHANG Jia-ming(College of Life Science,Nanjing Agricultural University,Nanjing 210059;Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crops Biology and Genetic Resources,Ministry of Agriculture,Haikou 571101;Hainan Institute of Tropical Agricultural Resources,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第4期938-944,共7页
Journal of Plant Genetic Resources
基金
海南省重点研发计划(ZDYF2018241)
中国热带农业科学院基本科研业务费专项资金(1630052019021,1630052016009)。
