摘要
建立了羊肚菌Morchella sextelata菌丝中单核苷类化合物的含量高效毛细管电泳测定方法。确定采用菌粉按照1∶30加水先超声后水浴的方法提取样品,最佳电泳条件为pH为9.4的50 mmol/L硼砂缓冲液以及1.0 mg/m L的β-环糊精为运行缓冲液,分离电压为25 kV,进样时间为8 s。在最佳电泳条件下,5种标准品在20 min内得到了分离,精密度试验中同一批次三次进样的标准品混合物迁移时间RSD均小于0.89%,峰面积RSD均小于2.9%;在稳定性试验中,5种标准品在8 h内迁移时间RSD均小于0.61%,峰面积RSD均小于4.73%。表明标准品在8 h内具有良好的稳定性。结果表明:该方法能够测定出羊肚菌中含有3-脱氧腺苷、腺苷和尿苷。通过建立标准曲线测得羊肚菌菌丝中3-脱氧腺苷含量为4.38 mg/g,腺苷含量为6.6 mg/g,尿苷含量为7.8 mg/g。3种单核苷类化合物的回收率在95.5~100.8%之间,且RSD小于4.9%(n=3)。表明该方法适用于羊肚菌Morchella sextelata菌丝中的单核苷类化合物的测定。
A new method using high performance capillary electrophoresis—diode array detector for the analysis of mononucleosides in the extracts from Morchella sextelata was described.It was determined that the dried mycelium was used to extract the sample according to the method that it extracted in 30 times water by Ultrasound first and then followed by water bath.In order to simultaneously determine five mononucleosides(3-deoxyadenosine,adenosine,guanosine,uridine andinosine),the conditions of high performance capillary electrophoresis(HPCE)were optimized.The optimum conditions were determined as follows:50 mmol/L borax buffer with pH9.4 andβ-cyclodextrin of 1.0 mg/m L as running buffer,separation voltage of 25 k V,injection time of 8 s.Under the optimum electrophoresis conditions,the five standards were separated in 20 min,and the RSD of migration time was less than 0.89%and the RSD of peak area was less than 2.9%.In the stability test,the RSD of the five standards in the 8 h migration time was less than 0.61%,and the RSD of peak area was less than 4.73%.It indicated that the sample had good stability within 8 h.The results showed that the method could be used to determine the content of 3 deoxyadenosine,adenosine and uridine in Morchella.The content of 3-deoxyadenosine in the mycelium of the morel was4.38 mg/g,the adenosine content was 6.6 mg/g,and the uridine content was 7.8 mg/g.The recoveries of the three mononucleoside compounds ranged from 95.5%to 100.8%,and the RSD was less than 4.9%(n=3).This method was suitable for the determination of mononucleoside compounds in Morchella sextelata hyphae.
作者
马晓颖
吕立涛
曹君
李跃
王红
MA Xiao-ying;LV Li-tao;CAO Jun;LI Yue;WANG Hong(Edible Fungus Institute,Liaoning Academy of Agricultural Sciences,Key Laboratory of Edible Fungi High Quality Cultivation,Liaoning Province,Shenyang 110161,China)
出处
《食品工业科技》
CAS
北大核心
2020年第15期237-243,共7页
Science and Technology of Food Industry
基金
辽宁省农业攻关及产业化(2017201005)
食药用菌栽培学科建设(2019DD072311)
辽宁省自然科学基金(201602419)
沈阳市应用基础研究(17-231-1-08)
清原县食用菌省级科技特派团(2020JH5/10100016)
沈阳于洪区食用菌省级科技特派团(2019JH5/10100009)。
关键词
高效毛细管电泳
羊肚菌
菌丝体
单核苷类化合物
high performance capillary electrophoresis
Morchella sextelata
mycelium
mononucleosides
