摘要
目的探究姜油酮对盐酸多柔比星诱导的慢性心力衰竭(CHF)大鼠的保护作用。方法腹腔注射盐酸多柔比星构建CHF大鼠模型,随机分为3组:模型组.姜油酮15 mg/kg组和姜油酮30 m/kg组,每组8只,另选8只作为正常对照组。采用超声检查仪检查各组大鼠收縮末期左心室内径(LVIDs)、舒张末期左心室内径(LVID4)、缩短分数(FS)和射血分数(EF)。HE染色观察心肌组织病理变化。蛋白质免疫印迹法(Westerm印迹)检测心肌细胞P62.L.C3-1和1C3-II蛋白。结果与模型组比较,姜油酮组LVIDd.LVIDs.LC3-1I1.LC3-1I/I水平均降低(P<0.05),EF、Fs.P62水平均升高(P<0.05);且姜油酮30 mg/kg组LVIDd、LVIDs.LC3-11、LC3-11/1水平低于姜油酮15 myg/kg组(P<0.05).EF、FS.P62水平高于姜油酮15 mg/kg组(P<0.05)。结论姜油酮对盐酸多柔比星诱导的CHF大鼠有--定保护作用,其机制可能与调节心肌细胞自噬蛋白有关。
Objective To investigate the protective efleet of zingerone on doxorubicin hydro-chloride-induced chronic heart failure(CHF)rats.Methods A rat model of CHF was established by intraperitoneal injection of doxorubicin hydochloride.The rats were randomly divided into three groups:model group,zingerone 15 mg/kg group and zingerone 30 mg/kg group.Ultrasound exarmi-nation was used to examine the left ventricular intermal diarmeler at end-systole(LVIDs),left ven-.tricular internal diameter at end-diastole(LVIDd),shortening fraction(FS),and ejection frac-tion(EF)of in the rats of each group.The pathological changes of myocardial tissue were observed by HE staining.The P62,LC3-I and LC3-II proteins of cardiomyocytes were detected by Westem botting.Results Compared with the model group,the levels of LVIDd,LVIDs,LC3.II,and LC3-1I/I in the zingerone group were lower(P<0.05),and the levels of EF,FS,and P62 were higher(P<0.05).The levels of LVIDd,LVIDs,LC3-II and LC3-1I/I were lower in zingerone 30 mg/kg group than in zingerone 15 mg/kg group(P<0.05),and EF,FS and P62 levels were higher than in zingerone 15 mg/kg group(P<0.05).Conclusion Zingerone posses-ses protective ffeet on doxorubicin-induced CHF rats,and its mechanism may be related to the reg-ulation of cardiomyocyte autophagy.
作者
王静
王念
刘宗波
陈姚
WANG Jing;WANG Nian;LIU Zongbo;CHEN Yao(The West China Hospital of Sichuan University,Chengdu,610041,China)
出处
《医学分子生物学杂志》
CAS
2020年第3期239-243,共5页
Journal of Medical Molecular Biology
基金
四川省科技计划项目(No.2019 YFS0529)。
关键词
慢性心力衰竭
姜油酮
多柔比星
自噬蛋白
chronie heart failure
zingerone
doxorubicin
aulophagy protein
