摘要
In this study, we aimed to examine whether curcumin exerted its anti-tumor effects by regulating miR-29 b/KDM2 A in cervical cancer cells. The cell viability, migration and invasion were estimated in HeLa cervical cancer cells treated with curcumin. The effects of microRNA-29 b(miR-29 b) on biological behaviors of HeLa SiHa cells were also assessed. Potential target genes of miR-29 b were predicted and confirmed using a luciferase reporter assay, and the effects of curcumin and miR-29 b on the PI3 K/AKT signaling pathway were analyzed. Curcumin treatment inhibited cell proliferation, migration and invasion of HeLa cells(P<0.05). The miR-29 b expression was promoted by curcumin treatment in HeLa cells(P<0.01), and miR-29 b depletion could restore the effects of curcumin on cell proliferation, migration and invasion of HeLa cells(P<0.05). KDM2 A was proved as a direct target gene of miR-29 b, and the activity of the PI3 K/AKT signaling could be regulated by curcumin and miR-29 b(P<0.05). All the data revealed that curcumin played a protective role in cervical cancer. The proliferation, migration and invasion of cervical cancer cells were inhibited by curcumin through the miR-29 b/KDM2 A/PI3 K/AKT pathway.
研究姜黄素通过调控miR-29b/KDM2A抑制宫颈癌细胞侵袭和迁移的作用机制。用姜黄素处理HeLa宫颈癌细胞,观察细胞活力、细胞迁移和细胞侵袭情况。我们还评估了micro RNA-29b (miR-29b)对HeLa SiHa细胞生物学行为的影响。利用荧光素酶报告基因实验预测和确认mi R-29b的潜在靶基因,分析姜黄素和mi R-29b对PI3K/AKT信号通路的影响。姜黄素对HeLa细胞的增殖、迁移和侵袭均有抑制作用(P<0.05)。在HeLa细胞中,姜黄素可促进miR-29b的表达(P<0.01),而mi R-29b的下调可恢复姜黄素对HeLa细胞增殖、迁移和侵袭的影响(P<0.05)。KDM2A被证实是mi R-29b的直接靶基因,而姜黄素和mi R-29b可以调控PI3K/AKT信号通路的活性(P<0.05)。姜黄素对宫颈癌具有有效的保护作用。姜黄素可通过mi R-29b/KDM2A/PI3K/AKT通路抑制宫颈癌细胞的增殖、迁移和侵袭。
