摘要
目的:本研究对犬经心脏脑组织灌流方法进行改进,以提高固定效果。方法:7只比格犬随机分为3组:对照组1只,经心脏灌流法3只,改进灌流法3只。使用改进灌流法的实验犬在麻醉后行全身肝素化,穿刺股动脉后,在透视引导下经股动脉将导管插至一侧颈内动脉作为流入道,开放右心耳作为流出道,并结扎下腔静脉及腹主动脉,先灌注生理盐水,后灌注4%多聚甲醛。灌注结束后取出脑组织进行HE染色与电镜观察。结果:相比传统经心脏灌流法,改进灌流法的脑组织细胞自溶现象明显减少,且更加省液、省时。结论:改进后的灌流方法提高了犬脑组织固定效果,是一种可行有效的大动物脑组织灌流固定方法。
Objective:This study aims to modify the transcardial perfusion method to improve the effects of brain fixation in canines.Methods:Seven beagle dogs were randomly divided into three groups:control group(n=1),transcardial perfusion group(n=3),and improved perfusion group(n=3).Canines in the improved perfusion group were anaesthetized and heparin was administrated.The femoral artery access was achieved,then a catheter was inserted into internal carotid artery under fluoroscopic guidance and constituted the inflow,and the right auricle was opened to act as an outflow access.After the ligation of inferior vena cava and abdominal aorta,the brains were perfused with saline followed by paraformaldehyde.Once the perfusion was accomplished,the brains were harvested for HE staining and transmission electron microscopy.Results:Compared with the transcardial perfusion method,the improved perfusion method can significantly reduce the occurrence of cell autolysis,and it is liquid-saving and time-saving.Conclusion:The improved perfusion method is more effective than transcardial perfusion in canine brain tissue fixation,and it is feasible for brain fixation in large animals.
作者
姜润浩
冷斌
王斌
邱凯
黄新鑫
刘圣
祖庆泉
JIANG Runhao;LENG Bin;WANG Bin;QIU Kai;HUANG Xinxin;LIU Sheng;ZU Qingquan(Department of Interventional Radiology,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第6期821-825,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81501565,81571777,81771794)。
关键词
犬
灌流固定
脑组织
介入放射
canines
fixation perfusion
brain
interventional radiology
