摘要
目的:探讨Max作用蛋白1-0(max action protein 1-0,Mxi1-0)在氧糖剥夺(oxygen-glucose deprivation,OGD)诱导SH-SY5Y细胞凋亡中的作用。方法:利用Western blot检测SH-SY5Y细胞在OGD条件下Mxi1-0和线粒体自噬相关蛋白LC3-Ⅱ、p62、Tom20的表达水平。Mxi1-0 siRNA转染SH-SY5Y细胞,以CCK-8法和Annexin V/PI染色法检测Mxi1-0在OGD诱导细胞凋亡中的作用。结果:OGD条件下,SH-SY5Y细胞中Mxi1-0的表达先上升后下降。OGD处理使线粒体自噬相关蛋白LC3-Ⅱ表达升高,p62和Tom20蛋白表达下降。OGD条件下,Mxi1-0沉默可抑制OGD诱导的线粒体自噬,增强OGD诱导的SH-SY5Y细胞凋亡。结论:Mxi1-0通过增强线粒体自噬拮抗OGD诱导的SH-SY5Y细胞凋亡。
Objective:This study aims to investigate the role of max action protein 1-0(Mxi1-0)in SH-SY5 Y cell apoptosis induced by oxygen-glucose deprivation(OGD).Methods:The expression levels of Mxi1-0 and mitophagy related proteins LC3-Ⅱ,p62,Tom20 in SH-SY5 Y cells under OGD conditions were detected by Western blot.Mxi1-0 siRNA was tranfected into SH-SY5 Y cells.The expression of Mxi1-0 and mitophagy related proteins(LC3-Ⅱ,p62,Tom20)were detected in SH-SY5 Y cells under OGD using Western blot.The role of Mxi1-0 in OGD-induced apoptosis was detected using CCK-8 method and Annexin V/PI staining.Results:The expression of Mxi1-0 increased firstly and then decreased in SH-SY5 Y cells after OGD treatment.The expression of mitophagy related protein LC3-Ⅱincreasesed and the expression of p62 and Tom20 proteins decreased after OGD treatment.Under OGD conditions,Mxi1-0 silence reduced mitophagy in SH-SY5 Y induced by OGD,and increase the apoptosis of SH-SY5 Y induced by OGD.Conclusion:Mxi1-0 antagonizes OGD induced apoptosis of SH-SY5 Y cells by enhancing mitochondrial autophagy.
作者
聂萍
胡圳圳
袁雅
吴伟玲
郑大同
NIE Ping;HU Zhenzhen;YUAN Ya;WU Weiling;ZHENG Datong(Department of Pediatrics,the Second Clinical Medical School,Nanjing Medical University,Nanjing 210011;Clinical Molecular Diognostic Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210003,China;Children’s Medical Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210003,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第6期816-820,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省妇幼健康科研项目(F201711)。
