摘要
目的建立人凝血酶原复合物中锑含量的测定方法。方法使用氢化物-原子吸收光谱法测定人凝血酶原复合物中锑的含量。结果锑含量在0-25.0 ng/ml范围内呈良好的线性关系,相关系数大于0.99;检出限为0.5533 ng/ml,定量限为1.844 ng/ml;加样回收率为95.93%-118.62%。结论本研究所建立方法简单、快速、灵敏度高,可用于测定人凝血酶原复合物锑的含量。
Objective To establish a method for the determination of antimony content in human prothrombin complex.Methods The content of antimony in human prothrombin complex was determined by hydride generation-atomic absorption spectrometry.Results The calibration curve of antimony was linear in the range of 0-25.0 ng/ml with correlation coefficient higher than 0.99.The detection limit was 0.5533 ng/ml and the quantitative limit was 1.844 ng/ml.The recoveries were 95.93%-118.62%.Conclusion The method is simple,rapid,accurate and sensitive,and can be suitable for the determination of antimony in human prothrombin complex.
作者
吴勰乾
韩杨
霍建富
连建科
任飞
WU Xieqian;HAN Yang;HUO Jianfu;LIAN Jianke;REN Fei(Department of Technical Centre,Shanxi Kangbao Biological Product CO.,Ltd,Changzhi 046000,China;Central Laboratory,Changzhi Medical College)
出处
《山西医科大学学报》
CAS
2020年第7期674-677,共4页
Journal of Shanxi Medical University
基金
长治医学院博士科研启动基金资助项目(BS1703)
山西省教育厅高校科技创新计划项目(2019L0684)。
关键词
人凝血酶原复合物
锑
氢化物-原子吸收光谱法
human prothrombin complex
antimony
hydride generation-atomic absorption spectrometry
