摘要
目的:研究沉默细胞分裂相关基因NUF2的表达对卵巢癌细胞侵袭及迁移的影响,并初步探讨其潜在的分子作用机制。方法:用基因表达谱数据动态分析(GEPIA)和实时荧光定量PCR(qRT-PCR)分别检测卵巢癌组织和细胞中NUF2的表达情况;将卵巢癌HEY、SKOV3细胞分为空白对照组、control siRNA(si-control)组和NUF2 siRNA(si-NUF2)组,用qRT-PCR检测各组细胞的NUF2 mRNA表达水平以验证转染效果;Transwell小室实验和细胞划痕实验检测沉默NUF2后各组细胞侵袭、迁移能力的改变;蛋白印迹法检测各组细胞中Notch通路相关蛋白Notch1、Hes1的表达水平。结果:NUF2在卵巢癌组织或细胞中均高表达,差异均有统计学意义(P<0.05);与空白对照组和sicontrol组相比,si-NUF2组细胞中NUF2 mRNA表达水平显著下降(P<0.05),侵袭、迁移细胞数明显减少(P<0.05),Notch1和Hes1蛋白表达降低。结论:沉默NUF2表达可抑制卵巢癌细胞侵袭、迁移能力,其分子作用机制与抑制Notch信号通路有关。
Objective:To investigate the role of cell division related gene NUF2 in the invasion and migration of ovarian cancer cells, and to explore the potential molecular mechanisms.Methods:Gene expression profiling interactive analysis(GEPIA) and real-time fluorescence quantitative PCR(qRT-PCR) were used to detect the expression of NUF2 in ovarian cancer tissues and cells respectively. Ovarian cancer cell lines HEY and SKOV3 were cultured, and were divided three groups of control, control siRNA(si-control) and NUF2 siRNA. qRT-PCR was used to identify NUF2 expression. Transwell chamber test and the cell scratch test were used to detect the abilities of cell invasion and migration after silencing NUF2. Western blotting was used to detect the expression of Notch pathway-related proteins.Results:The expressions of NUF2 in ovarian cancer tissues and cells were significantly higher than in the adjacent tissues and normal ovarian epithelial cells(P<0.05). The mRNA expression of NUF2 in ovarian cancer cells decreased after transected with NUF2 siRNA(P<0.05). Silencing NUF2 decreased the number of invasive and migrating cells(P<0.05). The expressions of Notch1 and Hes1 decreased significantly after NUF2 knockdown.Conclusion:Silencing NUF2 expression can inhibit the invasion and migration of ovarian cancer cells, and the underlying molecular mechanism is related to the inhibition of the Notch signaling pathway.
作者
任梦
赵晓婷
岳文涛
REN Meng;ZHAO Xiao-Ting;YUE Wen-Tao(Central Laboratory,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100026,China)
出处
《生物技术通讯》
CAS
2020年第3期269-274,共6页
Letters in Biotechnology
基金
首都临床特色应用研究重点项目(Z181100001718193)
首都医科大学附属北京妇产医院北京妇幼保健院中青年学科骨干培养专项(FCYY201818)。
