摘要
目的:探究人参与人及大鼠肠道菌群相互作用的特点及差异。方法:UPLC法测定人参水煎液与人、大鼠肠道菌群在厌氧条件下共培养24h前后皂苷Rg1、Re、Rb1、Rd的含量变化及代谢产物原人参二醇(PPD)、原人参三醇(PPT)的含量,同时宏基因测序分析人、大鼠肠道菌群结构。结果:人参中含有原人参三醇型皂苷Rg11.8132mg/g、Re 1.2944mg/g,原人参二醇型皂苷Rb11.5706mg/g、Rd 0.1426mg/g。与人源肠道菌共培养后,Re含量升高,其他皂苷含量降低,在共培养液中检测到代谢产物PPD(0.0340mg/g)和PPT(0.0143mg/g)的存在。与大鼠肠道菌共培养后,原人参二醇型、原人参三醇型皂苷含量均有轻微降低,有少量PPT(0.0110mg/g)的生成,但无PPD生成。另外,大鼠肠道菌群结构比人源结构多样性丰富,与人参共培养后,人源肠道菌中大肠杆菌属含量显著增多,大鼠肠道菌中芽孢杆菌数目减少,乳酸杆菌属水平显著增多。结论:在体外条件下,人参水煎液中人参皂苷会被人肠道菌群降解生成代谢产物PPD和PPT,但是大鼠中仅有PPT生成,存在种属差异。且人参对人源及大鼠肠道菌群结构的调整特点不同。
Objective:To explore the characteristics and differences of interaction of Ginseng Radix et Rhizoma with rat or human gut microflora.Methods:During experiments,the content of 4 kinds of saponins(Rg1,Re,Rb1 and Rd)in the incubating solution and the content of PPD and PPT of the metabolites were measured by UPLC method after the ginseng water decoction was incubated with the gut microflora of human or rat respectively under the anaerobic condition.At the same time,metagene sequencing was used to analyze the structural characteristics of human and rat intestinal flora.Results:The content of ginsenoside was Rg11.8132 mg/g,Re 1.2944 mg/g,Rb11.5706 mg/g,Rd 0.1426 mg/g in the Ginseng Radix et Rhizoma,respectively.After co-culture with human gut microflora,the content of ginsenosides Re was increased,the content of other 3 kinds of ginsenosides was significantly reduced.It was important that the contents of metabolites PPD and PPT were detected in the culture medium.The content was 0.0340 mg/g and 0.0143 mg/g,respectively.The 4 kind of ginsenosides content was slight decrease after co-culture with the rats’gut microflora.Moreover,a small amount of PPT(0.0110 mg/g)was formed,but no PPD.In addition,the structure of the rat gut microflora is more abundant than that of the human gut microflora.After co-culture with the ginseng solution,the content of Escherichia coli in the human intestinal bacteria is significantly increased,while the number of bacillus in the rat intestinal bacteria was decreased,the level of Lactobacillus was significantly increased.Conclusion:Ginsenosides can be degraded by human gut microflora to produce metabolites PPD and PPT,but only PPT in rats under the conditions of in vitro,there are significant different between species.Ginseng has different characteristics for the adjustment of human/rat intestinal flora structure.
作者
李寒冰
董贝贝
吴宿慧
阎茹玉
李根林
吕宁
何家欢
LI Han-bing;DONG Bei-bei;WU Su-hui;YAN Ru-yu;LI Gen-lin;LYU Ning;HE Jia-huan(Henan University of Chinese Medicine,Henan Zhongjing Recipe Health and Aging Industry Engineering Research Center,Zhengzhou 450046,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第6期3113-3118,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81503363)
河南省科技厅科技研发专项(No.192102310173)。
关键词
肠道菌群
人参
人参皂苷
代谢差异
Gut microflora
Ginseng Radix et Rhizoma
Ginsenoside
Metabolism difference
