摘要
目的:根据癌巢内肥大细胞、癌旁交界区的肥大细胞数量的变化及白介素-8的表达情况,确定和结直肠癌的关系。方法:收集80例结直肠癌患者术后标本,通过病理的免疫组化方法和链霉菌抗生物素蛋白—过氧化酶免疫组化染色超敏试剂盒检测,确定癌巢内和癌旁交界区的肥大细胞数量变化和白介素-8表达的变化情况。结果:80例结直肠癌患者,肿瘤细胞浸润深度与巢内的肥大细胞记数(MCC)比较,差异无统计学意义(χ2=2.342,P>0.05);与有无淋巴结转移比较,差异有统计学意义(χ2=6.857,P<0.05),但淋巴结转移的数量比较,差异无统计学意义(χ2=1.365,P>0.05);与有无远处脏器转移比较,差异无统计学意义(χ2=1.974,P>0.05);高分化腺癌和低分化腺癌比较,差异有统计学意义(χ2=6.352,P<0.05);黏液腺癌和腺癌比较,差异有统计学意义(χ2=6.965,P<0.05)。交界区的MCC与癌巢内MCC比较,差异有统计学意义(χ2=6.874,P<0.05),但交界区的MCC病理分期间比较,差异无统计学意义(χ2=2.643,P>0.05);黏液腺癌与腺癌比较,差异有统计学意义(χ2=6.432,P<0.05);低分化腺癌与高中分化腺癌比较,差异有统计学意义(χ2=5.453,P<0.05)。80例结直肠癌IL-8表达阳性44例(55%),评分(1.89±1.76)分。区域淋巴结转移病例IL-8阳性表达率及其评分明显高于未转移病例,差异有统计学意义(χ2=6.437,P<0.05);黏液腺癌与腺癌IL-8表达阳性率和评分比较,差异有统计学意义(χ2=6.763,P<0.05);结直肠癌的浸润深度、有无远处脏器转移、分化程度间比较,差异均无统计学意义(χ2值分别为1.054,1.965,0.984,P>0.05)。结论:结直肠癌患者恶性程度越高,癌巢内肥大细胞(MC)数量越多,预后越差,同时MC在癌周围聚集,去抑制的生长和扩散。IL-8是一种趋化因子,肿瘤的恶性程度越高,IL-8表达率越高,MC聚集越多,IL-8可能具有促进癌巢内血管生成以及肿瘤生长、扩散和转移等作用。
Objective:To study the relationship between mast cells(MC)and interleukin-8(ZL-8)and colorectal cancer,according to the number of mast cells and interleukin-8 expression in tumor nests and adjacent borderline areas.Methods:All 80 specimens of colorectal cancer patients were collected after surgery,and the changes in the number of mast cells and the expression of interleukin-8 were determined by immunohistochemistry and immunohistochemical staining kit of streptomyces antibiotin protein-peroxidase.Results:In 80 patients with colorectal cancer,the depth of tumor cell infiltration was not statistically significant compared with mast cell count(MCC) in the nest cancer(χ2=2.342,P>0.05).The number of mast cells in the cancer nest was statistically significant in patients with lymph node metastasis compared with no lymph node metastasis(χ2=6.857,P<0.05),but the number of lymph nodes metastasized was not statistically significant(χ2=1.365,P>0.05).There was no statistically significant difference between have and not distant organ metastasis(χ2=1.974 P>0.05).The comparison between highly differentiated adenocarcinoma and poorly differentiated adenocarcinoma was statistically significant(χ2=6.352 P<0.05).Comparison of mucinous adenocarcinoma and adenocarcinoma was statistically significant(χ2=6.965,P<0.05).There was significant statistical significance between MCC in the junction area and MCC in cancer nests(χ2=6.874,P<0.05),but no significant statistical significance between MCC in the junction area and pathological staging(χ2=2.643,P>0.05).There were statistically significant differences between mucinous adenocarcinoma and adenocarcinoma and between low-differentiated adenocarcinoma and high-differentiated adenocarcinoma(χ2=6.432,χ2=5.453,P<0.05).80 cases of colorectal cancer IL-8 express positive cases 44 cases(55%),the mean score of(1.89±1.76),in addition to the cases of regional lymph node metastasis,IL-8 positive expression rate and its score significantly higher than the transfer case (χ2=6.437,P<0.05) compared with mucous adenocarcinoma and adenocarcinoma IL-8 expression positive rate and score was statistically significant(χ2=6.763,P<0.05),IL-8 expression positive rate and score infiltration depth,and presence of distant organ metastasis of colorectal cancer,degree of differentiation were no significant statistical significance(χ2=1.054,χ2=1.965,χ2=0.984,P>0.05).Conclusion:The higher the degree of malignancy of colorectal cancer patients,the greater the number of mast cell in the cancer nests,and the worse the prognosis.Meanwhile,MC aggregates around the cancer to inhibit the growth and spread.IL-8 is a chemokine.The higher the malignant degree of tumor,the higher the expression rate of IL-8,the more MC aggregates,and IL-8 promotes angiogenesis,tumor growth,spread and metastasis in cancer nests.
作者
池澈
蔺春芳
冯小莉
韩秀芳
杨智文
邢晋梁
CHI Che;LIN Chunfang;FENG Xiaoli;HAN Xiufang;YANG Zhiwen;XING Jinliang(Datong Third People′s Hospital Affiliated to Shanxi Medical University,Datong 037000,China;The Second Hospital of Shanxi Medical University,Taiyuan 030001,China)
出处
《临床医药实践》
2020年第8期575-579,共5页
Proceeding of Clinical Medicine
