摘要
目的:观察虎杖痛风颗粒及其拆方对急性痛风性关节炎模型大鼠抗炎作用及对IL-6、TNF-α、PGE2表达的影响。方法:将大鼠随机分为正常组、模型组、虎杖痛风颗粒组、清热利湿组、通络组、秋水仙碱组。造模72h后取材,HE染色观察滑膜组织病理学变化,并采用Real-time PCR、ELISA技术检测大鼠关节滑膜组织及血清中IL-6、TNF-α、PGE2 mRNA和蛋白水平变化。结果:组织病理学提示虎杖痛风颗粒及其拆方均能降低大鼠关节滑膜组织炎症反应。与正常组比较,模型组IL-6、TNF-α、PGE2 mRNA和蛋白水平显著升高(P<0.05,P<0.01);与模型组比较,虎杖痛风颗粒组IL-6、TNF-α mRNA和蛋白水平显著降低(P<0.05,P<0.01),且能显著下调PGE2蛋白表达水平(P<0.05);清热利湿组IL-6 mRNA和蛋白水平显著降低(P<0.05,P<0.01),亦能显著下调PGE2蛋白表达水平(P<0.05);通络组显著下调IL-6、TNF-αmRNA表达水平(P<0.05);秋水仙碱组显著降低TNF-αmRNA、蛋白及IL-6蛋白表达水平(P<0.05,P<0.01)。结论:虎杖痛风颗粒可减轻急性痛风性关节炎模型大鼠炎症反应,可能与下调炎症因子IL-6、TNF-α的表达有关,对于PGE2的下调仅在蛋白质合成、分泌以及活化过程中实现。
Objective:To observe the anti-inflammatory effect of Huzhang Tongfeng Granules and its disassembled prescription on the expression of IL-6,TNF-α and PGE2 in rats with acute gouty arthritis.Methods:The rats were randomly divided into normal group,model group,Huzhang Tongfeng Granules group(Huzhang group),heat-clearing and dampnessremoving formula group,collateral-dredging formula group and colchicine group.After 72 h of modeling,the histopathological changes of synovial tissue were observed by HE staining.Real-time PCR and ELISA were used to detect the changes of IL-6,TNF-α and PGE2 mRNA and protein levels in the synovial tissues and serum of the rats.Results:Histopathology indicated that Huzhang Tongfeng Granules and its disassembled prescription could reduce the inflammation in synovial tissue of the joints in rats.IL-6,TNF-α and PGE2 m RNA and protein levels were significantly higher in the model group than in the normal group(P<0.05,P<0.01).Compared with the model group,IL-6 and TNF-αmRNA and protein levels in the Huzhang group were significantly decreased(P<0.05,P<0.01),and the expression level of PGE2 protein was significantly down regulated(P<0.05).IL-6 mRNA and protein level were significantly decreased in the heat-clearing and dampness-removing formula group(P<0.05,P<0.01),and the expression level of PGE2 protein was also significantly down regulated(P<0.05).In the collateral-dredging formula group,the expression levels of IL-6 and TNF-α mRNA were significantly decreased(P<0.05).Colchicine group significantly reduced the mRNA and protein levels of TNF-α and the protein level of IL-6(P<0.05,P<0.01).Conclusion:Huzhang Tongfeng Granules can meliorate the inflammatory response in rats with acute gouty arthritis,which may be related to the down-regulation of the expression of inflammatory factors,including IL-6 and TNF-α.The down-regulation of PGE2 is only achieved in the process of protein synthesis,secretion and activation.
作者
陈曦
卢怡
华亮
迮侃
杨滢瑶
王一飞
张明
李斌
周蜜
CHEN Xi;LU Yi;HUA Liang;ZE Kan;YANG Ying-yao;WANG Yi-fei;ZHANG Ming;LI Bin;ZHOU Mi(Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Yueyang Hospital of lntegrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第7期3631-3634,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81603627)
上海市临床重点专科建设项目—中医皮肤病科(No.shslczdzk05001)
上海市进一步加快中医药事业发展三年行动计划(2018年-2020年)[No.ZY(2018-2020)-FWTX-4010]。
