摘要
为了解不同培养模式对人参不定根的形态变化及有效物质积累的影响,试验利用显微镜对固体培养与生物反应器培养的人参不定根生长状态进行了观测并统计了培养过程中人参不定根的根长、根数和根粗变化,测定了不同培养模式的人参不定根中的活性物质含量。结果表明:不同培养模式下人参不定根的根长、根粗和根数均随培养天数的增加而增加,但不同时间段的相对生长速率不同,其中,根粗和根数的相对生长速率在固体培养基中培养18~24d时最高,为0.0410mm/d和0.2473个/d,根长的相对生长速率在生物反应器中培养20~25d时最高,为0.4967mm/d。培养40d后2种培养方式下培养的不定根中活性物质含量无明显差异,但生物反应器培养的不定根鲜重和干重分别达到91.86和10.73g/L,固体培养基中不定根鲜重和干重仅为10.38和1.37g/L,表明生物反应器培养更有利于人参不定根生物量的积累,可在短时间内大量获得所需植物材料。
In order to understand the morphological changes of Panax ginseng adventitious roots(ARs)and the effect of different culture patterns on the active substance accumulation in ARs,the growth state of P.ginseng ARs in solid medium and bioreactor were observed using a microscope in this study.The root length,root number,and root thickness of adventitious roots of ginseng were counted,and the content of active substances in ARs under different culture patterns was determined.The results showed that the root length,root thickness,and root number of adventitious root increased with the number of days of cultivation,but the relative growth rates were different at different periods.The relative growth rates of root thickness and root number were the highest when cultured in solid medium for 18-24 d,reached0.0410 mm/d and 0.2473 root/d.The relative growth rate of root length was highest when cultured in bioreactor for 20-25 dreached 0.4967 mm/d.There was no significant difference in the active substance contents in the ARs under the two cultivation methods after 40 dcultivation,but fresh weight(FW)and dry weight(DW)of ARs cultured in the bioreactor reached 91.86 and 10.73 g/L as well as FW and DW of ARs cultured in solid medium only reached 10.38 and 1.37 g/L.It suggested that bioreactor culture enhanced the biomass accumulation of P.ginseng ARs and could obtain lots of plant materials in a short time.
作者
郝悦君
孙浩丁
车成来
廉美兰
朴炫春
HAO Yuejun;SUN Haoding;CHE Chenglai;LIAN Meilan;PIAO Xuanchun(Agricultural College of Yanbian University,Yanji Jilin 133002,China)
出处
《延边大学农学学报》
2020年第2期14-20,共7页
Agricultural Science Journal of Yanbian University
基金
国家自然科学基金(31660080)。
关键词
人参
生物反应器
不定根
动态观测
活性物质
Panax ginseng
bioreactor
adventitious roots
dynamic observation
active substance
