摘要
目的建立基于QuEChERS与液相串联质谱(liquid chromatography tandem mass spectrometry,LC-MS/MS)联用技术快速测定蔬菜水果中氟吗啉和烯酰吗啉残留量的分析方法。方法样品经乙腈提取后,用QuEChERS法净化,Symmetry C18色谱柱分离,LC-MS/MS外标法定量。结果方法学验证,该方法具有较高的灵敏度,氟吗啉和烯酰吗啉的检出限为0.05μg/kg,定量限为0.1μg/kg;在0.1~25μg/kg浓度范围,线性相关系数大于等于0.9963,回收率在82.4%~106.3%之间,相对标准偏差为1.5%~8.8%,该方法具有较好的回收率和良好的再现性,满足国家相关标准要求。结论该方法处理过程快速简便,灵敏度高、专属性强。
Objective To establish a rapid method for the determination of flumorph and dimethomorph residues in fruits and vegetables by QuEChERS and liquid chromatography tandem mass spectrometry(LC-MS/MS).Methods After extracted by acetonitrile,the samples were purified by QuEChERS method,separated by symmetry C18 column and quantified by LC-MS/MS.Results The high sensitivity of this method was confirmed by methodology.The LOD and LOQ of flumorph and enolomorphine were 0.05μg/kg and 0.1μg/kg,and the linear correlation coefficients were greater than or equal to 0.9963 respectively in the concentration range of 0.1–25μg/kg.The recovery rate was 98.93%to 102.1%,and the relative standard deviation was 1.5%–8.8%.The method had good recovery and reproducibility,and met the requirements of relevant national standards.Conclusion This method has good linear relationship and stability,high recovery and accuracy,and meets the requirements of relevant national standards.
作者
王岩松
贺明睿
王冬妍
罗景阳
李兴全
袁帅
张宏宏
WANG Yan-Song;HE Ming-Rui;WANG Dong-Yan;LUO Jing-Yang;LI Xing-Quan;YUAN Shuai;ZHANG Hong-Hong(Shenyang food and Drug Inspection Institute,Shenyang 110124,China;College of Chenical Engineering Shenyang University of Chemical Technology,Shenyang 110142,China)
出处
《食品安全质量检测学报》
CAS
2020年第13期4455-4463,共9页
Journal of Food Safety and Quality
基金
辽宁省自然科学重点项目(20170520216)。
关键词
QUECHERS
液相-串联质谱法
氟吗啉
烯酰吗啉
蔬菜水果
QuEChERS
liquid chromatography tandem mass spectrometry
flumorph
dimethomorph
vegetables and fruits
