摘要
【目的】旨在成功建立FMDVC57BL/6小鼠的实验感染模型。【方法】采用体内和体外循环适应传代的方法,选取一株对C57BL/6小鼠不敏感FMDVO/HK/CHA/99MF4,将其在C57BL/6小鼠(体内)和胎猪肾原代细胞FPK(体外)进行多次循环适应传代。【结果】成功获得一株对C57BL/6小鼠敏感的FMDVO/HK/CHA/99MF4C5株。【结论】本研究成功建立了FMDV突变株感染C57BL/6小鼠的实验动物模型,为未来FMD疫苗效力的评估和致病性相关的研究奠定了基础。
[Objective]To successfully establish the C57 BL/6 mice experimental infection model for foot-and-mouth disease virus.[Methods]The foot-and-mouth disease virus O/HK/CHA/99 MF4 strain,which is insensitive to C57 BL/6 mice,was selected and continuously passaged in C57 BL/6 mice(in vivo)and fetal pig kidney cells(in vitro).[Results]We successfully obtained a foot-and-mouth disease virus mutant(O/HK/CHA/99 MF4 C5),which was sensitive to C57 BL/6 mouse.[Conclusion]This study successfully established C57 BL/6 mice model for a foot-and-mouth disease virus mutant strain,which lays a foundation for the evaluation of the efficacy of foot-and-mouth disease vaccine and the study on pathogenicity in the future.
作者
龚婷
黄书伦
查晶晶
孙普
马雪青
白兴文
袁红
曹轶梅
李坤
张婧
卢曾军
刘在新
李平花
Ting Gong;Shulun Huang;Jingjing Zha;Pu Sun;Xueqing Ma;Xingwen Bai;Hong Yuan;Yimei Cao;Kun Li;Jing Zhang;Zengjun Lu;Zaixin Liu;Pinghua Li(State Key Laboratory of Veterinary Etiological Biology,National Foot-and-Mouth Disease Reference Laboratory,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,Gansu Province,China;Henan University of Animal Husbandry and Economy,Zhengzhou 450000,Henan Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2020年第7期1413-1420,共8页
Acta Microbiologica Sinica
基金
“十三五”国家重点研发计划(2017YFD0501104)。
关键词
口蹄疫病毒
小鼠
细胞
循环传代
全序的测定与分析
Foot-and-mouth disease virus
mice
cell
cyclic passage
analysis of complete sequence
