摘要
目的研究胎膜组织、母血、脐带血中黑色素瘤缺乏因子2(AIM2)表达水平与胎膜早破(PROM)合并组织学绒毛膜羊膜炎(HCA)之间的关系,为感染性PROM的早期诊断提供参考。方法选取2018年5月至2019年5月就诊的PROM患者(研究组)120例及同期足月正常剖宫产孕妇(对照组)30例作为研究对象。胎盘娩出后取新鲜胎膜,根据胎膜组织病理检查结果将研究组进一步分为A组:未足月胎膜早破(PPROM)合并HCA;B组:足月胎膜早破(TPROM)合并HCA;C组:PPROM合并非HCA;D组:TPROM合并非HCA。免疫组化检测胎膜组织中AIM2蛋白表达水平,免疫荧光检测胎膜组织中AIM2和半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的表达,qRT-PCR检测胎膜组织中AIM2和caspase-1 mRNA的表达,ELISA法检测母血及脐带血中AIM2及白细胞介素(IL)-1β、IL-18的含量。结果研究A和B组胎膜组织中AIM2和caspase-1蛋白和mRNA的表达较对照组、研究C组、D组显著增加(P均<0.01);研究C组和D组表达较对照组稍增加,但差异无统计学意义(P均>0.05)。各组胎膜组织中AIM2与caspase-1蛋白表达呈一致性变化。研究A和B组母血及脐带血中AIM2、IL-1β及IL-18的含量较对照组、研究C组、D组显著增加(P<0.05,P<0.01);研究C和D组较对照组稍增加,但差异无统计学意义(P均>0.05)。结论AIM2、caspase-1的表达和IL-1β、IL-18的水平升高可能与PROM合并HCA有关,与非感染性的PROM关系不大。AIM2及其下游因子IL-1β和IL-18的检测对PROM合并HCA可能具有预测意义。
Objective To study the associations of absent in melanoma 2(AIM2)in the fetal membrane,maternal blood and cord blood with the premature rupture of fetal membranes(PROM)complicated by histologic chorioamnionitis(HCA)to provide reference for the early diagnosis of infectious PROM.Methods From May 2018 to May 2019,120 PROM patients(experimental group)and 30 normal full-term pregnant women receiving cesarean section(control group)were selected as study subjects.According to the pathological results of fresh fetal membranes after delivery,the experimental group was subdivided into PPROM combined with HCA group(group A),pre-premature rupture of fetal membranes(TPROM)combined with HCA(group B),PPROM with non-HCA group(group C)and TPROM with non-HCA group(group D).The expression levels of AIM2 protein,AIM2 and caspase-1 and mRNA levels of AIM2 and caspase-1 in fetal membranes were respectively detected by immunohistochemistry,immunofluorescence and qRT-PCR.ELISA was used to detect the levels of AIM2,IL-1βand IL-18 in maternal and umbilical blood.Results The protein and mRNA expressions of AIM2 and caspase-1 in group A and B increased compared with control group,group C and D(all P<0.01),and were similar between group C,D and control group(all P>0.05).The protein expressions of AIM2 and caspase-1 showed consistent changes among the four groups.The levels of AIM2,IL-1βand IL-18 in maternal blood and cord blood were significantly higher in group A and B than those in control group,group C and D(P<0.05,P<0.01),and there were no statistical differences in them between group C,D and control group(all P>0.05).Conclusions High expressions of AIM2 and caspase-1 and high levels of IL-1β,IL-18 may be related to PROM with HCA,but not to non-infectious PROM.AIM2 and its downstream IL-1βand IL-18 may have predictive significance for PROM with HCA.
作者
王倩
朱锦明
黄蕾
卢彩红
李颖慧
马春玲
WANG Qian;ZHU Jin-ming;HUANG Lei;LU Cai-hong;LI Ying-hui;MA Chun-ling(Graduate School of Xuzhou Medical University,Xuzhou,Jiangsu 221004,China;不详)
出处
《中国临床研究》
CAS
2020年第7期865-869,共5页
Chinese Journal of Clinical Research
基金
江苏省徐州市科技局科研项目(KC15SH022)。
