摘要
目的探讨白癜风患者肠道微生物菌群特点,分析肠道菌群变化与白癜风发病的关系,为临床治疗提供新思路。方法2017年4-12月,杭州市第三人民医院皮肤科收集30例白癜风患者及30例健康对照粪便标本,通过Roche/45高通量测序平台对其16S rRNA V3区进行定性分析;用宏基因组学对5例白癜风患者粪便及5例健康对照者粪便进行分析,明确其潜在调控通路。结果与健康对照比较,白癜风患者粪便中细菌物种相似,但白癜风患者肠道微生物多样性富集明显减少(P<0.05);在门水平上,变形杆菌和梭杆菌丰度明显降低;在属水平上,7个种属(拟杆菌、大肠杆菌志贺菌、罗氏囊菌、相炭疽杆菌、梭杆菌、柔膜细菌-RF9、普氏菌-7)丰度明显降低(P<0.05),4个种属(瘤胃球菌-1、瘤胃球菌科UCG、毛螺菌科、链球菌)明显增加(P<0.05);链球菌属及相炭疽杆菌在白癜风患者表达差异尤为明显,前者增加10.8倍,后者减少6.517倍。通过构建一个基于11个白癜风相关属的肠道微生物菌群的随机森林模型,显示该判别模型在ROC中AUC为0.89;宏基因组分析显示,白癜风相关菌群失调主要与免疫相关通路(如WNT通路、Notch通路等)、能量代谢、线粒体功能、氨基酸代谢(如苯丙氨酸代谢)通路相关。结论白癜风患者肠道微生态环境中细菌群落多样性与健康对照存在明显差异,其菌群失调可能参与白癜风的发病发展,补充益生菌可能有益于白癜风治疗。
Objective To study the characteristics of intestinal microflora in patients with vitiligo,and to analyze the relationship between the changes of intestinal microflora and the incidence of vitiligo,so as to provide new ideas for clinical treatment.Methods Fecal specimens from 30 patients with vitiligo and 30 healthy adults were collected and analyzed qualitatively by Roche/45 high-throughput sequencing platform.At the same time,macrogenomics was used to analyze the feces of 5 patients with vitiligo and 5 healthy adults to identify the potential regulatory pathways.Results The bacterial species in the feces of patients with vitiligo were similar to those of healthy people,but the intestinal microbial diversity of patients with vitiligo was significantly reduced(P<0.01);the abundance of Proteus and Clostridium was significantly reduced at phylum level;at genus level,7 of them were Bacteroides,Escherichia coli Shigella,Rochella,Bacillus anthracis,Clostridium clostridium,Jordani bacteria.The abundance of RF9 and Prunella-7 decreased significantly(P<0.01),while the abundance of 4 genera(Rumen Coccus-1,Rumen Coccus UCG,Trichomonas and Streptococcus)increased significantly(P<0.01).The expression of Streptococcus and Phase Anthraceae in vitiligo patients was significantly different:the former increased by 10.8 times,the latter decreased by 6.517 times,and an intestinal microorganism based on 11 vitiligo-related genera was constructed.The random forest model of bacterial flora showed that AUC of the discriminant model was 0.89 in ROC,and macrogenomic analysis showed that the disorders of vitiligo-related bacterial flora were mainly related to immune-related pathways(such as WNT pathway,Notch pathway),energy metabolism,mitochondrial function and amino acid metabolism(such as phenylalanine metabolism).Conclusions The diversity of bacterial community in intestinal microecological environment of vitiligo patients is significantly different from those in normal people.The imbalance of bacterial community may be involved in the pathogenesis and development of vitiligo.Supplementation of probiotics may be beneficial to the treatment of vitiligo.
作者
吴辛刚
鲍华烨
洪为松
许爱娥
Wu Xingang;Bao Huaye;Hong Weisong;Xu Ai'e(Department of Dermatology,Third People's Hospital of Hangzhou,Hangzhou 310009,China)
出处
《中华医学美学美容杂志》
2020年第3期242-245,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金(81472887,81773335,81602755)
浙江省基础公益研究计划项目(LGFl8H110002)。
