摘要
为明确吉林省公主岭和敦化的大豆根腐病镰孢菌种类,分离93份根腐病标样的病原真菌,利用ITS、TEF1-α、RPB2基因序列比对和多基因位点系统发育分析,结合形态学鉴定方法,鉴定出104株镰孢菌,共分4种,腐皮镰孢菌占64.42%,尖镰孢菌占33.65%,轮枝镰孢菌占0.96%,层出镰孢菌占0.96%。在公主岭罹病植株上分离鉴定出57株镰孢菌,腐皮镰孢菌占91.23%,尖镰孢菌占5.27%,轮枝镰孢菌占1.75%,层出镰孢菌占1.75%;在敦化罹病植株上分离鉴定出47株镰孢菌,腐皮镰孢菌占31.91%,尖镰孢菌占68.09%。结果表明:公主岭优势病原菌为腐皮镰孢菌,而敦化优势病原菌为尖镰孢菌。致病性试验结果表明,仅有3株腐皮镰孢菌对合丰55无致病性,其余101株镰孢菌对合丰55均有不同程度的致病性。
In order to identify the fusarium species associated with soybean root rot in Dunhua and Gongzhuling,93 samples of diseased plants with typical root rot symptom were collected to isolate and identify the species of Fusarium causing the disease in Gongzhuling and Dunhua, Jilin province. Sequence alignment carried out by ITS, TEF1-α and RPB2 genes, multiple-gene phylogenetic analysis and morphology were used to identify the pathogens. 104 strains of Fusarium classified in 4 species were identified, including F. solani(64.42%), F. oxysporum(33.65%), F. verticillioides(0.96%) and F. proliferaum(0.96%). 57 out of the 104 strains of Fusarium were isolated from the diseased plants in Gongzhuling, including F. solani(91.23%), F. oxysporum(5.27%), F. verticillioides(1.75%) and F. proliferaum(1.75%). The other 47 strains were isolated from Dunhua, including F. solani(31.91%) and F. oxysporum(68.09%). The results showed F. solani and F. oxysporum were respectively the dominant pathogen in Gongzhuling and Dunhua. Pathogenicity test showed that only 3 strains of F. solani had no pathogenicity to Hefeng 55, but the other 101 strains had different degrees of pathogenicity to Hefeng 55.
作者
桑永生
刘月
袁翠平
邱红梅
刘晓冬
王跃强
李玉秋
董英山
SANG Yong-sheng;LIU Yue;YUAN Cui-ping;QIU Hong-mei;LIU Xiao-dong;WANG Yue-qiang;LI Yu-qiu;DONG Ying-shan(College of Agriculture,Jilin Agricultural University,Changchun 130118,China;Jilin Academy of Agricultural Sciences,Changchun 130033,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2020年第3期442-450,共9页
Soybean Science
基金
吉林省农业科学院结余经费(统筹部分)(y81990401)
吉林省农业科技创新工程(C92070404)。
