摘要
目的探讨辛伐他汀联合阿仑膦酸钠干预对去卵巢大鼠骨质疏松骨代谢的影响。方法 60只雌性SD大鼠随机平均分为5组:假手术组、去势组、辛伐他汀组、阿仑膦酸钠组、联合药物组,首先构建去卵巢大鼠骨质疏松性模型。分别检测骨代谢相关生化指标、氧化应激生化指标和骨组织骨密度(bone mineral density,BMD),HE染色观察骨组织形态学。结果去势组大鼠血清Ca、P、SOD、CAT和骨组织BMD均较假手术组显著降低(P<0.05),辛伐他汀组、阿仑膦酸钠组、联合药物组上述指标均较去势组升高,以联合用药组升高最显著(P<0.05)。去势组大鼠血清ALP、BGP、PICP、TRAP、GLA、ICIP和MDA较假手术组均显著增高(P<0.05),辛伐他汀组、阿仑膦酸钠组、联合药物组上述指标较去势组均降低,以联合用药组降低最显著(P<0.05)。去势组股骨骨小梁明显稀疏,连接不完整,大量纤维组织,髓腔内大量空泡状脂肪细胞。联合药物组骨小梁数目明显增多,结构较完整,粗细均匀致密,连接成网状。结论辛伐他汀联合阿仑膦酸钠通过调节去卵巢大鼠骨代谢,抗氧化应激,增加骨密度,改善骨组织结构,发挥抗骨质疏松作用。
Objective To investigate the effect of simvastatin combined with alendronate on bone metabolism in ovariectomized osteoporosis rats.Methods Sixty female SD rats were randomly divided into 5 groups:sham operation group,castration group,simvastatin group,alendronate group,and combination group.The osteoporosis model was established in ovariectomized rats.Biochemical markers related to bone metabolism,biochemical indicators of oxidative stress,and BMD of bone tissue were examined.Bone histomorphology was observed with HE staining.Results The serum levels of Ca,P,SOD,CAT,and BMD in the ovariectomized group were significantly lower than those in the sham operation group(P<0.05).The above indexes in simvastatin group,alendronate group,and combination group were higher than those in the ovariectomized group.The most significant increase was in the combination group(P<0.05).The serum levels of ALP,BGP,PICP,TRAP,GLA,ICIP,and MDA in the ovariectomized group were significantly higher than those in the sham operation group(P<0.05).The above indicators in the simvastatin group,the alendronate group,and the combination group were lower than those in the ovariectomized group.The most significant reduction was in the combination group(P<0.05).The castrated trabecular bone in the ovariectomized group was obviously sparse,the connection was incomplete,and a large amount of fibrous tissue and more vacuolar fat cells were found in the medullary cavity.The number of trabecular bone in the combined drug group increased significantly.The structure was relatively completed,the thickness was uniform and dense,and the mesh was connected.Conclusion Simvastatin combined with alendronate inhibits bone metabolism,anti-oxidative stress,increases bone mineral density,improves bone structure,and exerts anti-osteoporosis effects.
作者
吴琼
WU Qiong(Department of Joint Sports Medicine,Shengli Oilfield Central Hospital,Dongying 257000,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2020年第7期1049-1053,共5页
Chinese Journal of Osteoporosis
关键词
辛伐他汀
阿仑膦酸钠
骨代谢
氧化应激
骨质疏松
simvastatin
alendronate sodium
bone metabolism
oxidative stress
osteoporosis
