摘要
[目的]观察程序性死亡受体1(PD1)和程序性死亡配体1(PD-L1)在结肠癌患者组织中的表达,并分析两者表达水平与患者临床病理特征及预后的相关性关系。[方法]采用免疫组织化学法检测112例结肠癌患者结肠癌组织及癌旁组织中PD-1、PD-L1蛋白表达水平,分析结肠癌组织PD-1、PD-L1蛋白表达水平与患者临床病理特征、预后的相关性,同时分析影响结肠癌患者生存期的多元因素。[结果]结肠癌组织PD-1、PD-L1蛋白表达水平均明显高于癌旁组织(P<0.05);结肠癌组织PD-1、PD-L1蛋白表达水平与TNM分期、分化程度、浸润深度、脉管侵犯及淋巴结转移等临床病理特征存在明显的相关性(P<0.05);PD-1高表达组(n=67)3年累计生存率(67.2%)及中位生存时间(20.2个月)均明显低于PD-1低表达组(88.9%)及中位生存时间(31.6个月)(P<0.05)。PD-L1高表达组3年累计生存率(71.0%)及中位生存时间(21.5个月)均明显低于PD-L1低表达组(92.0%)及中位生存时间(32.2个月)(P<0.05);TNM分期晚、分化程度低、浸润深度深、脉管浸润、淋巴结转移、PD-1蛋白和PD-L1蛋白表达阳性均为影响结肠癌患者预后生存期的独立危险因素(P<0.05)。[结论]结肠癌组织PD-1、PD-L1蛋白表达水平与TNM分期、分化程度、浸润深度、脉管侵犯及淋巴结转移等临床病理特征存在明显的相关性,可作为预测结肠癌患者预后的重要指标。
[Objective]To observe the expression of programmed death 1(PD-1)and programmed death ligand-1(PD-L1)in the tissues of colon cancer patients,and analyze the correlation between their expression levels and the clinicopathological characteristics and prognosis.[Methods]The protein expression levels of PD-1 and PD-L1 in colon cancer tissue and tissue adjacent to carcinoma of 112 patients with colon cancer were detected by immunohistochemical method and the protein expression levels of PD-1 and PD-L1 were compared.The relationship of PD-1 and PD-L1 protein expression and the clinical pathological features was analyzed.The relationship of protein expression levels in the colon cancer tissue between PD-1 and PD-L1 was analyzed.The relationship between protein expression of PD-1 and PD-L1 and the prognosis of patients with colon cancer was analyzed,as well as the multiple factors of colon cancer survival was analyzed.[Results]The protein expression of PD-1 and PD-L1 in colon cancer tissues were significantly higher than that in para-carcinoma tissues(P<0.05).The expression levels of PD-1 and PD-L1 in colon cancer tissues were significantly correlated with the clinicopathological features such as TNM stage,degree of differentiation,depth of infiltration,vascular invasion and lymph node metastasis(P<0.05).The 3-year cumulative survival rate(67.2%)and median survival time(20.2 months)in the high-expression PD-1 group were significantly lower than those in the low-expression PD-1 group(88.9%)and the median survival time(31.6 months)(P<0.05),and the 3-year cumulative survival rate(71.0%)and median survival time(21.5 months)in the high-expression PD-L1 group were significantly lower than those in the low-expression PD-L1 group(92.0%)and the median survival time(32.2 months)(P<0.05).Late TNM stage,low differentiation,deep infiltration,vascular infiltration,lymph node metastasis,positive PD-1 protein expression and positive PD-L1 protein expression were all independent risk factors affecting the prognosis and survival of patients with colon cancer(P<0.05).[Conclusion]The expression levels of PD-1 and PD-L1 proteins in colon cancer tissues are significantly correlated with the clinicopathological features such as TNM stage,differentiation degree,invasion depth,vascular invasion and lymph node metastasis,which can be used as an important indicator to predict the prognosis of patients with colon cancer.
作者
黄健
任莉
张绍斌
彭泽
HUANG Jian;REN Li;ZHANG Shao-bin;PENG Ze(Department of Surgery,Jinyintan Hospital of Wuhan,Wuhan 430011,China;Department of No.4InternalMedicine,the Red Cross Hospital of Wuhan,Wuhan 430015,China)
出处
《中国中西医结合消化杂志》
CAS
2020年第7期499-503,共5页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
