摘要
利用紫外-可见吸收光谱、氢核磁共振、基质辅助激光解析电离飞行时间质谱、红外光谱、热重分析等方法分析了七元瓜环(Q[7])、八元瓜环(Q[8])与N-苄基氨基嘌呤(NBAP)的主客体作用模式,并进行了自组装包结物的细胞毒性测试和细胞成像研究。实验结果表明Q[7,8]与NBAP分别以不同的作用模式自组装形成了1∶1的包合物。NBAP完全进入Q[8]空腔所形成包结配合物的热稳定性强于Q[7]-NBAP配合物的稳定性。两种包结物的形成均降低了NBAP的细胞毒性,细胞成像结果进一步验证了两种包结物以不同包结模式完成了自组装。
Host-vip interaction of cucurbit[n]urils Q[7]and Q[8]with N-benzylaminopurine(NBAP)were investigated by using UV-Vis absorbance,~1HNMR,MALDI-TOF,IR and TGA methods.The cytotoxicity and imaging of self-assembly inclusion complex in cells were verified.The result indicated that 1∶1 inclusion complex of Q[7,8]with NBAP were formed by different self-assembly model.Thermal stability of inclusion complex Q[8]-NBAP stronger than that of Q[7]-NBAP complex.The formation of inclusion complex reduced the cytotoxicity of NBAP,furthermore,the different self-assembling models of the inclusion complex were confirmed by using cell images.
作者
李春荣
孟铁宏
刘仕云
姜艳萍
胡先运
LI Chun-rong;MENG Tie-hong;LIU Shi-yun;JIANG Yan-ping;HU Xian-yun(Qiannan Medical College for Nationalities,Duyun 558000,China)
出处
《化学试剂》
CAS
北大核心
2020年第7期852-857,共6页
Chemical Reagents
基金
贵州省自然科学基金资助项目(黔科合基础[2019]1301)
黔南民族医学高等专科学校基金资助项目(QNYZ201706,QNYZ201902)
黔南州科技计划项目(黔南科合社字(2017)19号)。
关键词
瓜环
自组装
包结物
NBAP
细胞成像
cucurbit[n]urils
self-assembly
inclusion complex
NBAP
cell imaging
