摘要
采用旋光法建立D-阿洛酮糖的快速检测方法,并对方法进行验证。确定旋光法快速定量检测D-阿洛酮糖的条件,并对方法的线性范围、准确度、精密度以及重复性进行验证。尽管D-阿洛酮糖的比旋光度受温度影响,在10~40℃范围内,随温度升高而升高。但是在同一温度条件下,0.01~0.3 g/mL浓度范围内,D-阿洛酮糖浓度与旋光值具有良好的线性关系(线性回归方程为y=3.77384x+0.01935,相关系数(R^2)为0.99972)。随机测定3个不同浓度D-阿洛酮糖溶液的旋光值,通过回归方程计算得到理论浓度,将理论浓度与实际浓度进行单因素方差分析,不存在显著性差异(p>0.05),说明方法具有较好的准确度。计算平行样品的相对标准偏差(RSD),发现精密度实验和重复性实验的RSD值均小于5%。因此,方法具有较好的精密度和重复性。方法在0.01~0.3 g/mL浓度范围内线性良好,且具有较好的准确度、精密度和重复性,能够作为一种快速、简便、准确的定量检测D-阿洛酮糖方法。
A rapid detection method was established for D-psicose by polarimetry,and the method was verified.The experimental conditions were determined and the linear range,accuracy,precision and repeatability of the method were verified.The results showed that the specific rotation of D-psicose was affected by temperature.In the range of the temperature(10~40℃),the specific rotation of D-psicose increased with the increasing of the temperature.However,at the same temperature conditions,there was a good linear relationship in the range of 0.01~0.3 g/mL between the concentration of D-psicose and the optical rotation(the regression equation:y=3.77384x+0.01935,R^2=0.99972).The optical rotation values of D-psicose solutions with three concentration gradients were randomly measured,and the theoretical concentrations of D-psicose solutions were calculated by the regression equation.One-way analysis of variance results(p>0.05)showed that there was no significant difference between the theoretical concentration and the actual concentration,indicating that the method has a good accuracy.The precision and repeatability of the method were evaluated and it turned out that the relative standard deviation(RSD)was less than 5%.Therefore,the method has better precision and repeatability.The linearity of the method was well in the concentration range of 0.01~0.3 g/mL,and the method has good accuracy,precision and repeatability.It is a rapid,simple and accurate method for the quantitative determination of D-psicose.
作者
邢敏钰
冉淦侨
殷红
韩姗姗
张红艳
张强
XING Min-yu;RAN Gan-qiao;YIN Hong;HAN Shan-shan;ZHANG Hong-yan;ZHANG Qiang(Bio-Agriculture Institute of Shaanxi,Xi’an 710043,China;Enzyme Engineering Research Center of Shaanxi Province,Xi’an 710600,China)
出处
《化学试剂》
CAS
北大核心
2020年第7期822-827,共6页
Chemical Reagents
基金
陕西省科学院科技计划项目(2017K-13、2019K-09)。
