摘要
目的观察凉血祛风颗粒对变应性鼻炎(AR)大鼠模型IL-4、IFN-γ表达的影响。方法SPF级Wistar大鼠50只随机分为空白对照组、模型对照组、低剂量凉血祛风颗粒组(9.45 g/kg)、高剂量凉血祛风颗粒组(18.9 g/kg)、氯雷他定组(0.9 mg/kg),每组10只。除空白对照组外,其余四组采用卵白蛋白(OVA)腹腔注射致敏、双侧鼻腔滴鼻激发制备AR大鼠模型。于第22天起,连续灌胃给药14天后处死大鼠,取血清和鼻黏膜组织,观察一般情况及鼻部症状评分;运用组织病理学方法观察鼻黏膜形态学改变;运用ELISA检测血清中IL-4、IFN-γ含量。结果与空白对照组比较,模型对照组干预后鼻部症状评分及IL-4含量升高,IFN-γ含量降低(P<0.05,P<0.01);与模型对照组比较,低、高剂量凉血祛风颗粒组及氯雷他定组鼻部症状评分及IL-4含量降低,IFN-γ含量升高(P<0.05,P<0.01)。HE染色显示,模型对照组大鼠的鼻黏膜损坏,大量炎性细胞浸润,腺体增生;各药物干预组较模型对照组病理变化明显减轻。结论凉血祛风颗粒可以有效缓解AR大鼠鼻痒、喷嚏、清涕等症状,减轻鼻部炎症反应,其机制可能是与抑制IL-4和增强IFN-γ的表达有关。
Objective To observe the effect of Liangxue Qufeng Granule(LQG)on the expression of IL-4 and IFN-γin allergic rhinitis(AR)rat model.Methods Totally 50 SPF Wistar rats were randomly divided into blank control group,model control group,low dose LQG group(9.45 g/kg),high dose LQG group(18.9 g/kg),and loratadine group(0.9 mg/kg),10 in each group.AR rat models were prepared by intraperitoneal injection of ovalbumin(OVA)for sensitization and bilateral intranasal challenge in the other four groups except blank control group.On the 22 nd day,the rats were sacrificed after gavage for 14 days.The serum and nasal mucosa were collected to observe the general condition and nasal symptom score.The morphological changes of nasal mucosa were observed by histopathological method,and the contents of IL-4 and IFN-γin serum were detected by ELISA.Results Compared with the blank control group,the nasal symptom score and IL-4 content increased,the content of IFN-γdecreased after intervention in the model control group(P<0.05,P<0.01).Compared with the model control group,the nasal symptom score and IL-4 content decreased and IFN-γcontent increased in the low and high dose LQG groups and loratadine group(P<0.05,P<0.01).HE staining exhibited that rats in the model control group showed damaged nasal mucosa and a great number of inflammatory cells infiltrated,glandular hyperplasia;drug intervention groups were significantly alleviative pathological changes compared with model control group.Conclusions LQG could effectively relieve nasal itching,sneezing,clear nasal discharge and other symptoms of AR rats,and alleviate nasal inflammation.The mechanism may be related to the inhibition of IL-4 and the enhancement of IFN-γexpression.
作者
温馨
王海
邢鑫鑫
WEN Xin;WANG Hai;XING Xin-xin(School of Basic Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040;The Second Department of Pediatrics,The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2020年第7期842-845,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
黑龙江中医药大学研究生创新基金项目(No.2017036)。
