摘要
MYB家族是调节花青素合成最为关键的转录因子成员,通过前期转录组测序发现,影响花青素的MYB转录因子中MYB108基因在不同时期表达具有差异,其对花青素生物合成调节作用未见报道。本研究成功克隆了MYB108基因,鉴定其ORF为891 bp,含有296个氨基酸,理论等电点(pI)为6.30,属不稳定蛋白,具有SANT/Myb结构域,鉴定是MYB转录因子家族基因。红皮梨色泽受到花青素含量的影响,本研究意在为今后验证其基因与花青素之间的关系提供先决条件。
The MYB family is the most important member of transcription factors that regulate anthocyanins synthesis. Through early transcriptome sequencing, it was found that MYB108 gene in the MYB transcription factors affecting anthocyanins had different expression in different periods, and its regulation effect on anthocyanin biosynthesis was not reported. The MYB gene was successfully cloned and identified as a MYB108 transcription factor family gene with an ORF of 891 bp, pI of 6.30, containing 296 amino acids, and it’s an unstable protein with a SANT/Myb domain. The color of red pear was affected by the anthocyanin content. The purpose of the study was to provide a prerequisite for further verification of the relationship between the gene and the anthocyanin.
作者
孟义淳
曲柏宏
王甜元
白金月
杜昱彤
王玥
MENG Yichun;QU Baihong;WANG Tianyuan;BAI Jinyue;DU Yutong;WANG Yue(College of Agriculture,Yanbian University,Yanji 133000,China)
出处
《东北农业科学》
北大核心
2020年第2期60-63,共4页
Journal of Northeast Agricultural Sciences
基金
国家自然科学基金(31160389)。
