摘要
随着用于监测食源性致病菌和暴发调查的分子分型技术迅猛发展,全基因组测序(whole genome sequencing,WGS)正逐渐显露出其重要性。在食品贸易全球化背景下,急需建立食源性致病菌与人类感染间联系的详尽认识,从而精确监测并减少其发生。在该方面,WGS的精确性明显优于之前的分析工具。本文主要以单核细胞增生李斯特菌(Listeria monocytogenes)为例,阐述食源性致病菌监测情况和感染暴发调查现状,强调WGS在食源性疾病溯源方面的价值,概述用于数据生成和分析的技术,着重介绍WGS在全球食源性致病菌分型领域的实际应用进展,并对其未来所面临的挑战进行展望。
With the rapid development of molecular typing techniques for monitoring foodborne pathogens and outbreak investigations,whole genome sequencing(WGS)is gradually revealing its importance.In the context of the globalization of food trade,it’s urgent to establish details of the links between foodborne pathogens and human exposure in order to accurately monitor and reduce their occurrence.The accuracy of WGS is significantly better than prior analysis tools in the aspect.In this paper,we take Listeria monocytogenes as example to expound the monitoring of foodborne pathogens and the investigation of infection outbreaks,emphasizing the value of WGS in trace-back of foodborne diseases.The technologies for data generation and analysis are summarized,the practical application progress of WGS in the worldwide foodborne pathogen typing is emphasized,and the challenges in the future are prospected.
作者
李晓然
张若鸿
杨洋
崔生辉
郭云昌
LI Xiaoran;ZHANG Ruohong;YANG Yang;CUI Shenghui;GUO Yunchang(College of Food Science and Technology,Hebei Normal University of Science and Technology,Hebei Qinhuangdao 066600,China;China National Institutes for Food and Drug Control,Beijing 100050,China;China National Center for Food Safety Risk Assessment,Beijing 100022,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2020年第3期339-344,共6页
Chinese Journal of Food Hygiene
基金
河北省自然科学基金面上项目(H2017407007)
河北省高等学校科学技术研究青年基金项目(QN2016256)
秦皇岛市科技支撑项目(201701B041)
河北科技师范学院博士研究启动基金项目(2015YB010)。
关键词
食源性致病菌
暴发
全基因组测序
全球数据共享平台
Foodborne pathogens
outbreak
whole genome sequencing
global data sharing platform
