摘要
目的建立基于气相色谱-质谱(gas chromatography-mass spectrometry, GC-MS)的靶向代谢组学方法进行海鸭蛋真伪鉴别。方法采用气相色谱-质谱法,对海鸭蛋和笼养鸭蛋中的脂肪酸种类和含量进行分析检测。进一步应用化学计量学进行海鸭蛋与笼养鸭蛋的差异分析,筛选出可用于海鸭蛋真伪鉴别的标志物。结果海鸭蛋中的omega-3脂肪酸(Ω-3)在总脂肪酸中的占比高于笼养鸭蛋,差异有统计学意义(t=23.58,P<0.05)。进一步通过主成分分析区分了海鸭蛋和笼养鸭蛋,通过正交偏最小二乘判别分析筛选出对海鸭蛋和笼养鸭蛋分类贡献最大的二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid, DHA),EPA仅在海鸭蛋中检出,DHA在海鸭蛋中的含量高于笼养鸭蛋。结论 EPA和DHA可以作为标志物进行海鸭蛋的真伪鉴别。
Objective A targeted metabolomics based on gas chromatography-mass spectrometry(GC-MS) was established for the authentication of sea duck eggs. Methods In this study, gas chromatography-mass spectrometry was used to characterize the fat acid profiles of sea duck eggs and cage duck eggs. Chemometrics was used to analyze the difference between sea duck eggs and cage duck eggs to screen the markers that could be used to identify the authenticity of sea duck eggs. Results Omega-3 fatty acids(Ω-3) in sea duck eggs account for a higher proportion of total fatty acids than cage duck eggs, and there were significant differences(t=23.58,P<0.05). Sea duck eggs and cage duck eggs were distinguished by principal component analysis. Eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) were screened by orthogonal partial least squares discriminant analysis that contributed the most to the difference of sea duck eggs and cage duck eggs. EPA was detected only in sea duck eggs, and the content of DHA in sea duck eggs was higher than that of cage duck eggs. Conclusion This study showed that EPA and DHA can be used as a reliable marker to authenticate sea duck eggs.
作者
张琪
董旭阳
许秀丽
杨旭升
贾玮
张峰
ZHANG Qi;DONG Xuyang;XU Xiuli;YANG Xusheng;JIA Wei;ZHANG Feng(Institute of Food Safety,Chinese Academy of Inspection and Quarantine,Beijing 100176,China;Beijing Forestry University,Beijing 100083,China;School of Food and Biological Engineering,Shaanxi University of Science and Technology,Shaanxi Xi’an 710021,China;Northeast Agricultural University,Heilongjiang Harbin 150038,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2020年第3期250-256,共7页
Chinese Journal of Food Hygiene
基金
国家重点研发计划项目(2017YFC1601600)
国家“万人计划”科技创新领军人才项目。
关键词
代谢组学
海鸭蛋
气相色谱-质谱
脂肪酸
真伪鉴别
Metabolomics
sea duck eggs
gas chromatography-mass spectrometry
fatty acids
authentication
