摘要
简化基因组测序(Reduced-representation Genome Sequencing,RRGS)是在二代高通量测序技术上,通过限制性内切酶对基因组进行打断,对基因组特定区域进行高通量测序,得到大量遗传多态性标签序列,进而展现整个基因组序列特征的技术。该方法建库过程简单,费用较低,能有效降低基因组复杂度。目前简化基因组测序已广泛应用在观赏植物分子标记开发、群体遗传及系统发生学、高密度遗传图谱构建、数量性状定位等研究中。综述了简化基因组测序技术原理及其在观赏植物中的研究进展,并讨论了研究中所存在的问题及对其发展前景进行了展望。
Reduced-representation Genome Sequencing(RRGS)was based on the second-generation high-throughput sequencing technology in which the genome was interrupted by restriction endonuclease and a large number of genetic polymorphism tag sequences were obtained by high-throughput sequencing of specific regions of the genome,and then revealing the characteristics of the entire Genome sequence.This method has the advantages of simple process of library building,cost-effective,and can effectively reduce the complexity of genome.At present,RRGS has been widely used in the development of molecular markers,population genetics and phylogeny,construction of high-density genetic map and quantitative characteristics localization of ornamental plants.In this paper,the technical principle of RRGS and its research progress in ornamental plants were reviewed,the problems and development prospects were also discussed.
作者
熊燕
张金柱
董婕
王力
高鹏
姜琬
车代弟
XIONG Yan;ZHANG Jinzhu;DONG Jie;WANG Li;GAO Peng;JIANG Wan;CHE Daidi(College of Horticulture,Northeast Agricultural University,Harbin 150030,China;Institute of Natural Resources and Ecology,Heilongjiang Academy of Sciences,Harbin 150040,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2020年第6期1194-1202,共9页
Acta Horticulturae Sinica
基金
黑龙江省自然科学基金项目(QC2017025)
东北农业大学“学术骨干”基金项目
黑龙江省院所基本应用技术研究专项(ZNBZ2019ZR04)。
关键词
简化基因组测序
观赏植物
高通量测序
SNP
reduced-representation genome sequencing
ornamental plant
high-throughput sequencing
SNP
