摘要
为研究花椰菜抗黑腐病的分子机制,本试验利用Illumina测序平台的RNA-Seq技术对花椰菜黑腐病感病品种Y1-2和抗病品种EC-247接种黑腐病菌5 d后的样本进行高通量转录组分析,采用RSEM软件计算基因表达量,并在此基础上筛选两组样本间的差异表达基因。结果显示,抗、感品种间差异表达基因共3 195个,其中上调表达基因1 424个,下调表达基因1 771个。通过GO和KEGG进行基因功能显著性富集分析发现,差异表达基因主要富集于苯丙烷类代谢、次级代谢产物合成、逆境响应、MAPK信号通路等途径。本研究结果可为解析花椰菜与黑腐病互作机制、挖掘黑腐病抗病基因提供参考。
In order to study the molecular mechanism of cauliflower resistant to black rot and to explore the related functional genes,the samples of susceptible material Y1-2 and resistant material EC-247 after inoculated with black rot strain for 5 days were selected as materials to perform high-throughput transcriptome sequencing by means of RNA-Seq technology of Illumina sequencing platform.The gene expression level was calculated using RSEM software,and the differentially expressed genes between the two materials were screened out.The results showed that there were 3 195 differentially expressed genes in the two materials,including 1 424 up-regulated genes and 1 771 down-regulated genes.Significant enrichment analysis of gene function by GO and KEGG revealed that differentially expressed genes were mainly enriched in phenylpropane metabolism,secondary metabolite synthesis,adversity response,MAPK signaling pathway and other pathways.The results of this study provided references for elucidating the interaction mechanism between cauliflower and black rot,and mining the resistance genes of black rot.
作者
姚玉荣
霍建飞
郝永娟
贲海燕
王万立
Yao Yurong;Huo Jianfei;Hao Yongjuan;Ben Haiyan;Wang Wanli(Tianjin Kerun Agricultural Technology Co.,Ltd./State Key Laboratory of Vegetable Germplasm Resources Innovation,Tianjin 300384,China;Tianjin Plant Protection Institute,Tianjin 300384,China)
出处
《山东农业科学》
2020年第7期1-6,共6页
Shandong Agricultural Sciences
基金
蔬菜种质创新国家重点实验室开放课题(201802)
天津市自然科学基金项目(18JCQNJC14900)。
关键词
花椰菜
黑腐病
转录组
差异表达基因
Brassica oleracea L.var.botrytis
Xanthomonas campestris pv.campestiss
Transcriptome
Differentially expressed gene
