摘要
目的研究藏药普尔那不同部位不同提取物的体外抗耐药菌和抗肿瘤活性。方法对藏药普尔那茎和叶分别采用无水乙醇、乙酸乙酯进行成分提取。采用K-B纸片法、比浊法及平板划线法,评价不同提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌活性。MTT法检测不同提取物对人肝癌细胞系HepG2细胞、人宫颈癌细胞系HeLa细胞的抑制率。结果37℃培养18 h后,普尔那叶无水乙醇提取物(100.00 mg/mL)对MRSA(1×10^7cfu/mL)抑制作用最强(5.00 mm),最低抑菌浓度(MIC)为1.25 mg/mL,最低杀菌浓度(MBC)为5 mg/mL。高浓度(500.00μg/mL)普尔那叶乙酸乙酯提取物对人肝癌细胞系HepG2细胞、人宫颈癌细胞系HeLa细胞均表现出最佳的抑制活性。结论普尔那叶乙醇提取物对MRSA体外抑耐药菌效果更佳,而普尔那叶乙酸乙酯提取物抗肿瘤活性更好。
Objective To study on anti-drug resistant bacteria and antitumor activity of different parts from different parts of Tibetan medicine Artemisia vestita in vitro.Methods Stem and leaf of Artemisia vestita were extracted with absolute ethanol and ethyl acetate respectively.The antibacterial activity of different extracts on Methicillin-resistant Staphylococcus aureus(MRSA)was evaluated by K-B paper method,turbidimetric method and flat line marking method.The MTT method was used to detect the inhibition rate of different extracts on human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells.Results After incubation at 37℃ for 18 h,the Artemisia vestita leaf absolute ethanol extract(100.00 mg/mL)had the strongest inhibitory effect(5.00 mm)on MRSA(1×10^7 cfu/mL),and the lowest inhibitory concentration(MIC)was 1.25 mg/mL,the minimum germicidal concentration(MBC)was5 mg/mL.Highconcentration(500.00μg/mL)Artemisia vestita leaf ethyl acetate extract showed the best inhibitory activity against human hepatoma cell line HepG2 cells and human cervical cancer cell line HeLa cells.Conclusion Artemisia vestita leaf ethanol extract has better anti-drug resistance to MRSA in vitro,while Artemisia vestita leaf ethyl acetate extract has better antitumor activity.
作者
李磊
崔鹤蓉
王鹏龙
陈静
米玛
雷海民
王成祥
LI Lei;CUI Herong;WANG Penglong;CHEN Jing;MI Ma;LEI Haimin;WANG Chengxiang(College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;Department of Respiratory,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Academic Affairs Office,Tibetan Traditional Medicine College,Tibet Autonomous Region,Lasa 850000,China)
出处
《中国医药导报》
CAS
2020年第20期17-20,共4页
China Medical Herald
基金
西藏自治区科技厅重点科研计划项目。
关键词
藏药
普尔那
耐甲氧西林金黄色葡萄球菌
抗耐药菌
抗肿瘤
Tibetan medicine
Artemisia vestita
Methicillin-resistant Staphylococcus aureus
Anti-drug resistant bacteria
Antitumor
