摘要
本试验旨在研究黄曲霉毒素B1(AFB1)和T-2毒素联合使用对肉鸡生长性能、器官指数、血清抗氧化指标和生化指标的影响,同时评价吸附剂的脱毒效果。选取1日龄健康爱拔益加(AA)肉仔鸡448羽,采用2×2双因子完全随机试验设计,随机分为4个组,每组8个重复,每个重复14羽(公母各占1/2)。对照组饲喂基础饲粮,Ⅰ组在基础饲粮中添加0.25%吸附剂,Ⅱ组在基础饲粮中添加0.2 mg/kg AFB1+1.0 mg/kg T-2毒素,Ⅲ组在基础饲粮中添加0.25%吸附剂+0.2 mg/kg AFB1+1.0 mg/kg T-2毒素。对照组和Ⅰ组为空白组,Ⅱ组和Ⅲ组为霉菌毒素组。试验期42 d。结果表明:与空白组相比,1)添加霉菌毒素显著提高肉鸡1~21日龄时的料重比(F/G)(P<0.05),显著降低22~42日龄时的末重和平均日增重(ADG)(P<0.05);添加吸附剂显著提高22~42日龄时的末重和平均日采食量(ADFI)(P<0.05)。2)添加霉菌毒素显著提高肉鸡21日龄时的脾脏指数(P<0.05),显著降低42日龄时的法氏囊指数(P<0.05);添加吸附剂缓解了42日龄时的法氏囊萎缩,但效果不显著(P>0.05)。3)添加霉菌毒素显著降低肉鸡21日龄时的血清过氧化氢酶(CAT)活性和42日龄时的血清总蛋白(TP)含量(P<0.05),显著提高21日龄时的血清谷丙转氨酶(ALT)活性(P<0.05);添加吸附剂对血清抗氧化能力改善效果不显著(P>0.05),显著降低21日龄时的血清碱性磷酸酶(ALP)活性(P<0.05),显著提高42日龄时的血清白蛋白(ALB)含量(P<0.05)。综上所述,吸附剂可有效缓解AFB1和T-2毒素对肉鸡生长性能、器官指数和血清生化指标带来的负面影响,可作为一种有效脱毒剂添加到肉鸡饲粮中。
This experiment was conducted to investigate the effects of combination of aflatoxin B1(AFB1)and T-2 toxin on growth performance,organ indexes,serum antioxidant indexes and biochemical indexes of broilers,and to evaluate the detoxification effects of adsorbent.Four hundred and forty-eight one-day-old healthy Arbor Acres(AA)broilers were randomly divided into 4 groups with 8 replicates per group and 14 broilers per replicate(males and females in half)by a 2×2 two factor completely randomized experiment design.Broilers in control group were fed a basal diet,that in groupⅠwere fed the basal diets supplemented with 0.25%adsorbent,that in groupⅡwere fed the basal diets supplemented with 0.2 mg/kg AFB1+1.0 mg/kg T-2 toxin,and the others in groupⅢwere fed the basal diets supplemented with 0.25%adsorbent+0.2 mg/kg AFB1+1.0 mg/kg T-2 toxin.Control group and groupⅠwere blank groups,and groupⅡand groupⅢwere mycotoxin groups.The experiment lasted for 42 days.The results showed as follows:compared with blank groups,1)supplemented with mycotoxin significantly increased the feed gain ratio(F/G)of broilers at 1 to 21 days of age(P<0.05),and significantly decreased final body weight and average daily gain(ADG)at 22 to 42 days of age(P<0.05);supplemented with adsorbent significantly increased final body weight and average daily feed intake(ADFI)at 22 to 42 days of age(P<0.05).2)Supplemented with mycotoxin significantly increased spleen index of broilers at 21 days of age(P<0.05),and significantly decreased bursa of Fabricius index at 42 days of age(P<0.05);supplemented with adsorbent alleviated bursa of Fabricius atrophy at 42 days of age,but the effect was not significant(P>0.05).3)Supplemented with mycotoxin significantly decreased serum catalase(CAT)activity of broilers at 21 days of age and serum total protein(TP)content at 42 days of age(P<0.05),and significantly increased serum alanine aminotransferase(ALT)activity at 21 days of age(P<0.05);supplemented with adsorbent had no significant effects on improvement of serum antioxidant capacity(P>0.05),significantly decreased serum alkaline phosphatase(ALP)activity at 21 days of age(P<0.05),and significantly increased serum albumin(ALB)content at 42 days of age(P<0.05).In conclusion,adsorbent can effectively alleviate the negative effects of AFB1 and T-2 toxin on growth performance,organ indexes and serum biochemical indexes of broilers,so it can be added to broiler’s diets as an effective detoxification agent.
作者
田莎
孙鑫东
宋泽和
吴媛媛
陈宇光
贺喜
TIAN Sha;SUN Xindong;SONG Zehe;WU Yuanyuan;CHEN Yuguang;HE Xi(College of Animal Science and Technology,Hunan Engineering Research Center of Poultry Production Safety,Hunan Co-Innovation Center of Animal Production Safety,Engineering Research Center of Feed Safety and Efficient Use of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;Trouw Nutrition Technology Co.,Ltd.,Beijing 100089,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2020年第7期3143-3153,共11页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家重点研发计划政府间国际科技创新合作重点专项(2018YFE0101700)
湖南农业大学重大科研项目暨创新团队培育工程资助项目(17PYXM04)
湖南农业大学“双一流”建设项目(SYL201802015)。
