摘要
【目的】探明干旱胁迫下,云南省主栽马铃薯品种合作88号、丽薯6号、青薯9号和宣薯2号抗氧化系统的生理响应机制,为抗旱马铃薯品种筛选及抗旱栽培与育种提供理论依据。【方法】采用人工模拟干旱胁迫的方式,研究干旱胁迫对马铃薯叶片抗氧化防御系统的影响。设置PEG6000浓度0%(CK)、15%、20%、25%和30%5个处理,测定不同处理后马铃薯叶片中的过氧化氢(H2O2)、可溶性蛋白(SP)、类胡萝卜素(Car)、还原型抗坏血酸(AsA)、还原型谷胱甘肽(GSH)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽过氧化物酶(GPX)和谷胱甘肽还原酶(GR)含量。【结果】干旱胁迫下,合作88号、丽薯6号、青薯9号和宣薯2号4个品种均对干旱胁迫作出响应,随干旱胁迫程度增加,4个马铃薯品种中过氧化氢含量呈上升趋势,合作88号过氧化氢(H2O2)积累量最大。在干旱胁迫条件下,4个马铃薯品种SP、AsA、GSH、APX、DHAR和GR、Car含量均显著高于对照(PEG6000浓度0%),其中PEG6000浓度为25%时,马铃薯叶片抗坏血酸(AsA)和谷胱甘肽(GSH)活性达峰值,而类胡萝卜素(Car)含量在30%浓度胁迫下酶活达峰值, APX、DHAR在PEG浓度20%时含量最高。【结论】马铃薯可通过抗氧化的酶促及非酶促系统的协同作用来清除活性氧,4个主栽马铃薯品种中,合作88号抗氧化防御系统对干旱胁迫的响应程度较其他品种强。
【Objective】The physiological response mechanism of the antioxidant system of main potato varieties Hezuo No.88,Lishu No.6,Qingshu No.9 and Xuanshu No.2 in Yunnan province under drought stress was explored,which provided theoretical basis for drought-resistant potato varieties selection and cultivation.【Method】The effects of drought stress on antioxidant defense system of potato leaves were studied by artificial simulation of natural drought stress.Five treatments of PEG6000 concentration 0 %(CK),15 %,20 %,25 % and 30 % were set,and the contents of hydrogen peroxide(H2O2),soluble protein(SP),carotenoid(Car),reduced ascorbic acid(AsA),reduced glutathione(GSH),ascorbic acid peroxidase(APX),monodehydroascorbic acid reductase(MDHAR),dehydroascorbic acid reductase(DHAR),glutathione peroxidase(GPX) and glutathione reductase(GR) in potato leaves were measured.【Result】Under drought stress,4 varieties of Hezuo No.88,Lishu No.6,Qingshu No.9 and Xuanshu No.2 all responded to drought stress.With the increase of drought stress,the content of hydrogen peroxide in the four potato varieties showed an increasing trend,and the accumulation of hydrogen peroxide(H2O2) in Hezuo No.88 was the largest.Under drought stress,the contents of SP,AsA,GSH,APX,DHAR,GR and Car of four potato varieties were significantly higher than those of the control(PEG6000 concentration of 0 %).Among them,under drought stress with PEG6000 concentration of 25 %,the activity of ascorbic acid(AsA) and glutathione(GSH) in potato leaves reached the peak,while the enzyme activity of carotenoid(Car) contents reached the peak under the stress of 30 %,the content of APX and DHAR was the highest when PEG concentration was 20 %.【Conclusion】Potato can resist drought by removing active oxygen through the synergistic action of antioxidant enzymatic and non-enzymatic systems.The response of the antioxidant defense system to drought stress of main potato varieties Hezuo No.88,Lishu No.6,Qingshu No.9 and Xuanshu No.2 in Yunnan province,Hezuo No.88 was stronger than that of others.
作者
任家慧
杨淼
王玥
黄钰
李润
胡锦飙
肖关丽
REN Jia-hui;YANG Miao;WANG Yue;HUANG Yu;LI Run;HU Jin-biao;XIAO Guan-li(College of Agronomy and Biotechnology,Yunnan Agricultural University,Yunnan Kunming 650201,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第6期1158-1164,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31760519)
国家重点研发计划(2018YFD0200703)。
关键词
马铃薯
干旱胁迫
抗氧化响应
Potato
Drought
Antioxidant defense system
