摘要
成簇的规律间隔的短回文重复序列/相关核酸酶9(CRISPR-Cas9)是存在于细菌和古生细菌中的一种抵御外源基因入侵的适应性免疫反应系统。与传统的锌指核酸酶和转录激活样因子效应物核酸酶基因编辑技术相比,CRISPR-Cas9具有操作简便、高效且脱靶效率低的优点。近年来,该项技术已在构建基因编辑肿瘤细胞模型、肿瘤治疗及治疗机制研究中得到广泛关注。本文就其在肿瘤细胞模型建立、肿瘤治疗及机制方面的研究应用进行综述,旨在为CRISPR/Cas9的临床前研究提供一定的理论依据与参考,为基因治疗提供基础。
CRISPR-Cas9 is an adaptive immune system of bacteria and archaea that resists the invasion of foreign genes.Compared with the traditional zincfinger nucleases and transcription activator-like effector nuclease gene editing technologies,CRISPR-Cas9 has the advantages of simple operation,high efficiency and low off-target efficiency.In recent years,this technique has been widely used in the construction of gene editing tumor cells.In this paper,the application of CRISPR-Cas9 in the construction of gene-editing tumor cells were reviewed,aiming to provide theoretical basis for the preclinical study of CRISPR-Cas9.
作者
陈燕
刘芷兮(综述)
肖洪涛(审校)
Chen Yan;Liu Zhixi;Xiao Hongtao(Department of Pharmacy,Sichuan Cancer Hospital&Institute,Sichuan Cancer Center,School of Medicine,University of Electronic Science and Technology of China,Chengdu 610041,Sichuan,China;Personalized Drug Therapy Key Laboratory of Sichuan Province,Chengdu 610072,Sichuan,China)
出处
《肿瘤预防与治疗》
2020年第6期542-548,共7页
Journal of Cancer Control And Treatment
基金
四川省科技厅项目(编号:20CXTD0043,2020JDTD0029)
四川省卫健委干部保健科研课题(编号:2019-018)
成都市药学会-赞邦药学科研基金资助课题(编号:2019-05)。
