摘要
目的探讨微小RNA-499(miR-499)、微小RNA-16(miR-16)、肌红蛋白(MYO)与常规心肌损伤标志物的关系及在急性心肌梗死(AMI)早期诊断中的价值。方法选取2017年2月至2019年12月本院收治的134例AMI患者(AMI组)及体检中心134例健康人群(对照组)。比较两组常规心肌损伤标志物[肌钙蛋白I(cTnI)肌酸激酶同工酶(CK-MB)]表达、miR-499 mRNA、miR-169 mRNA、MYO表达,采用Pearson分析miR-499、miR-16、MYO与cTnI、CK-MB相关性,采用接收者操作特征(ROC)曲线及ROC下面积(AUC)分析各指标在AMI早期诊断中的价值。结果发病2 h时,AMI组CK-MB组高于cTnI组,差异无统计学意义(P>0.05);发病6、12 h,AMI组cTnI、CK-MB均高于对照组(P<0.05);发病2、6、12 h时,AMI组miR-499 m RNA、miR-16 mRNA、MYO高于对照组(P<0.05);miR-499 m RNA与miR-16 mRNA、MYO呈正相关;miR-16 m RNA与MYO呈正相关(P<0.05);cTnI与miR-499、miR-16、MYO呈正相关(P<0.05);CK-MB与miR-499、miR-16、MYO呈正相关(P<0.05);诊断早期AMI的AUC:miR-499为0.751,截断值>1.37,miR-16的AUC为0.800,截断值>1.87,MYO的AUC为0.731,截断值>60.14μg/L(P<0.05)。结论 miR-499、miR-16、MYO在AMI发病早期即升高,各指标间呈正相关,并与cTnI、CK-MB呈正相关,可作为AMI早期诊断的标志物。
Objective To explore the relationship between microRNA-499(miR-499),microRNA-16(miR-16),myoglobin(MYO)and conventional myocardial injury markers,and to evaluate the value in the early diagnosis of acute myocardial infarction(AMI). Methods A total of 134 AMI patients(the AMI group)and 134 healthy people(the control group)in the medical examination center from February 2017 to December 2019 were enrolled. The levels of conventional cardiac injury markers[troponin I(cTnI),creatine kinase isoenzyme(CK-MB)],miR-499 mRNA,miR-169 m RNA,and MYO were measured and compared between the two groups. Pearson was used to analyze the correlation between miR-499,miR-16,MYO and cTn I,CK-MB,and the receiver operating characteristic(ROC)curve and area under ROC(AUC)were used to analyze the value of each index in the early diagnosis of AMI. Results At 2 h after the onset,there was no significant difference in cTnI and CK-MB between the AMI group and the control group(P>0.05). At 6 and12 h after onset,cTnI and CK-MB in the AMI group were higher than those in the control group(P<0.05). At2 h,6 hours,and 12 hs after onset,miR-499 m RNA,miR-16 mRNA,and MYO in the AMI group were higher than those in the control group(P<0.05). miR-499 m RNA was positively correlated with miR-16 m RNA and MYO. miR-16 mRNA was positively correlated with MYO(P<0.05). c TnI was positively correlated with miR-499,miR-16,and MYO(P<0.05). CK-MB was positively correlated with miR-499,miR-16,and MYO(P<0.05). AUC for early diagnosis of AMI:miR-499 was 0.751,cut-off value was >1.37,miR-16 had AUC of 0.800,cut-off value >1.87,MYOs AUC was 0.731,cut-off value was > 60.14 μg/L(P<0.05).Conclusions miR-499,miR-16,and MYO increased early in the onset of AMI,and were positively correlated with each index and cTnI and CK-MB. Therefore,those indicators could be used as a marker for early diagnosis of AMI.
作者
李刚
朱春凯
郑培明
LI Gang;ZHU Chunkai;ZHENG Peiming(Department of clinical laboratory,henan provincial people's hospital,Zhengzhou,Henan,China,450003)
出处
《分子诊断与治疗杂志》
2020年第6期728-732,共5页
Journal of Molecular Diagnostics and Therapy
基金
河南省科技厅重点项目(281803692)。
