摘要
目的通过网络药理学探究青风藤治疗类风湿性关节炎(RA)的药理机制,为RA的临床治疗提供理论依据。方法检索时间为建库至2018年12月,基于TCMSP、TCMID数据库搜集青风藤成分及靶点,通过Cytoscape软件构建成分-靶点网络;通过TTD、Drug Bank数据收集RA相关靶点,在STRING数据库构建靶点互作(PPI)网络。在Genomics数据库分析药物和疾病共有靶点,并利用Cytoscape软件中Cluego插件分析共同靶点的信号通路,最后构建成分-靶点-通路网络图并分析。结果青风藤发挥治疗RA的成分可能是拉兹马宁碱、千金藤啶碱、青藤碱等;发挥治疗作用的靶点可能包括Jun激酶(JUN)、维甲酸X受体(RXRA)、过氧化物酶体增殖活化受体γ(PPARG)等;关键作用通路包括PPAR通路、脂代谢调控、PTEN基因转录、核细胞受体及转录等13条。结论青风藤通过多组分、多靶点、多通路发挥镇痛消炎、减轻骨破坏等作用,对RA的治疗具有一定的价值。
Objective To explore the pharmacological mechanism of Caulis Sinomenii in the treatment of rheumatoid arthritis(RA)through network pharmacology,and to provide theoretical basis for the clinical treatment of RA.Methods The retrieval time was from the establishment of the database to December 2018.Based on the TCMSP and TCMID database,the components and targets of Caulis Sinomenii were collected,and the component-target network was constructed through the software of Cytoscape.RA related targets were collected through TTD and Drug Bank data,and the target interaction(PPI)network was constructed in the STRING database.The common target of drugs and diseases was analyzed in Genomics database,and the signal pathway of common target was analyzed by using Cluego plugin in the software of Cytoscape.Finally,the component-target-pathway network diagram was constructed and analyzed.Results The therapeutic components of Caulis Sinomenii in the treatment of RA might be 16-epi-Isositsirikine,Stepholidine,Sinomenine,etc.;the therapeutic targets might include Jun proto-oncogene(Jun),Retinoid X receptor alpha(RXRA),peroxisome proliferative activated receptor gamma(PPARG),etc.;the key pathways included PPAR pathway,lipid metabolism regulation,PTEN gene transcription,nuclear cell receptor and transcription,etc..Conclusion Caulis Sinomenii can relieve pain and inflammation and reduce bone destruction through multi components,multi targets and multiple pathways,which has a certain value for the treatment of RA.
作者
丁文聪
韩隆胤
钱凯
林昌松
DING Wencong;HAN Longyin;QIAN Kai;LIN Changsong(Department of Rheumatology and Immunology,the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou 510000,China;the First Clinical School,Guangzhou University of Chinese Medicine,Guangdong Province,Guangzhou 510000,China)
出处
《中国医药导报》
CAS
2020年第17期15-20,共6页
China Medical Herald
基金
国家自然科学基金资助项目(81573930)。
关键词
网络药理学
青风藤
类风湿性关节炎
成分-靶点-通路网络图
Network pharmacology
Caulis Sinomenii
Rheumatoid arthritis
Component-target-pathway network diagram
