摘要
聚合酶链反应(PCR)在动植物源掺杂鉴别、转基因成分和致病微生物等食品安全检测领域成为日趋重要的检测技术。从传统PCR、荧光PCR到数字PCR,PCR技术逐渐从定性分析、半定量分析发展到准确定量,不仅提升了准确度和检测效率,同时也扩展了食品检测范围,使食品安全的监管更加精细化。该文总结了近5年来数字PCR在食品安全检测中的研究进展,比较了传统PCR、荧光定量PCR和数字PCR的相关标准制订情况,列举、讨论了数字PCR在不同食品安全领域检测中的技术进展和存在的问题,并对数字PCR未来发展方向进行了展望。
Polymerase chain reaction(PCR)has become a more and more important detection technique for identification of animal sources,plant sources,genetically modified organisms(GMOs)and microorganisms in food safety detection field.The PCR technique evolving from conventional PCR(cPCR)to fluorescent PCR(qPCR)and digital PCR(dPCR),has gradually been applied in the qualitative,relative quantification and absolute quantification due to its ability to detect small amounts of nucleic acid in raw food materials,while dPCR,is especially of great advantage both in efficiency and accuracy compared with other methods.The latest research progresses of dPCR in food safety detection in recent five years are summarized in this paper,the relevant standards of cPCR,qPCR and dPCR in food analysis are compared,and the technical advances and problems of dPCR in different food safety fields are discussed.Finally,The future development of dPCR technique is anticipate.
作者
张宜文
赵海波
吴红
马康
ZHANG Yi-wen;ZHAO Hai-bo;WU Hong;MA Kang(Beijing Institute of Metrology,Beijing100021,China;National Institution of Metrology,Beijing100013,China)
出处
《分析测试学报》
CAS
CSCD
北大核心
2020年第5期672-680,共9页
Journal of Instrumental Analysis
基金
国家重大研发计划项目(2018YFF0212800,2018YFF0212801)。
