摘要
为探讨LiCl/DMSO溶剂体系对稻草化学组成溶出行为的影响,以球磨稻草为原料,分别选取稻草的秆和叶部位,通过LiCl/DMSO溶剂体系处理,测定再生前后的秆和叶部位的化学成分,采用碱性硝基苯氧化来分析木质素的结构单元变化。结果表明:球磨时间越长,木质素和高聚糖的再生得率会降低,球磨原料中灰分的再生不受球磨操作条件影响;稻草秆的再生能力高于叶,其中木质素和高聚糖的再生得率比叶的高,灰分的保留则较低。稻草的秆和叶经过LiCl/DMSO溶剂体系处理可促进木质素的溶出,处理后的秆更易于进行酶水解。经球磨后的木质素缩合程度增加,球磨改进了硝基苯氧化环境,其反应的均相性得到提高。再生酶水解后,各球磨部位缩合程度有所增加。其中从球磨1.0~2.0h的叶和秆分离出的木质素能代表相应原料中不同部位的原木质素。
In order to investigate the dissolution behavior of chemical component of rice straw and the feasibility of lignin isolation based on LiCl/DMSO solvent system,ball milled rice straw,was taken as study object.The internode and leaf of ball milled rice straw were treated with LiCl/DMSO solvent system,and general chemical composition analysis was conducted.The contents of structural unit of lignin were investigated by alkaline nitrobenzene oxidation.The results showed that:The longer the milling time,the lower the regeneration rate of lignin and polysaccharide;The regeneration of ash in the milling material was not affected by the operating conditions of ball milling;The regeneration ability of the internode sample was higher than that of the leaf,and the regeneration rates of lignin and polysaccharide were higher than that of the leaf,but the ash retention was lower;The ball milled rice straw samples treated by LiCl/DMSO solvent system could promote the dissolution of lignin,and the internode sample treated by LiCl/DMSO solvent system was easier to be hydrolyzed by enzyme.The results from nitrobenzene oxidation showed that the condensation degree of lignin increased after milling,and the condensation degree of lignin in leaf sample was higher than that in internode.After regeneration and enzymatic hydrolysis,the condensation degree of both ball mill samples increased.The lignin of leaf and internode,which was isolated from rice straw with ball milling for 1.0-2.0 h,could represent the original lignin in different parts of the raw material.
作者
吴文娟
王博伟
何贤
闫雪晴
邹春阳
WU Wenjuan;WANG Bowei;HE Xian;YAN Xueqing;ZOU Chunyang(Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing Forestry University,Nanjing 210037,China)
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2020年第6期46-56,共11页
Journal of China Agricultural University
基金
国家自然科学基金青年基金(21704045)
江苏省高等学校大学生创新创业训练计划(201910298034Y)
南京林业大学大学生创新训练计划(2018NFUSPITP673)。
关键词
木质素
LiCl/DMSO
溶解再生
稻草
酶水解
lignin
LiCl/DMSO
dissolution and regeneration
rice straw
enzymatic hydrolysis
