摘要
目的通过对中国范围内分布的巴尔通体进行系统发育多样性分析,了解我国巴尔通体分布种类情况,可为临床诊断提供参考。方法下载GenBank中2000-2019年公布的中国范围内的巴尔通体gltA基因序列,采用CLUSTAL X 2.0进行多序列比对确定变异体,再在NCBI中在线采用Blastn策略同源性搜索与各变异体相似性最高且是有效发表种的gltA基因序列,作为参考序列,然后利用MEGA 7.0进行系统发育分析,构建系统发育树。结果中国的巴尔通体大致可归为25个种类,其中有11个种类可对人类致病,分别为B.henselae、B.quintana、B.elizabethae、B.clarridgeiae、B.grahamii、B.melophagi、B.rattimassiliensis、B.rochalimae、B.tribocorum、B.vinsonii、B.washoensis;还有27个变异体可能是潜在的新种。云南、黑龙江、新疆巴尔通体种类最丰富。结论中国巴尔通体种类丰富,可致人类患病的种类也较多,还有一些潜在新种,尤以云南、黑龙江、新疆等地的多样性最高,可为一些不明原因疾病的诊断提供参考。
Objective To analyze the phylogenetic diversity of Bartonella in China,and provide reference for clinical diagnosis. Methods The China-wide Bartonella genus gltA gene sequence published in GenBank from 2000 to 2019 were downloaded for analysis. CLUSTAL X 2.0 was used for multiple sequence alignment to determine variants,and Blastn strategy online in NCBI was used to search for the gltA gene sequences with the highest similarity to each variant were used as reference sequences. Finally,phylogenetic analysis was performed using MEGA 7.0 to construct a phylogenetic tree.Results Bartonella in China could be roughly classified as 25 species. Among which,11 species were pathogenic to humans,namely B.henselae,B.quintana,B.elizabethae,B.clarridgeiae,B.grahamii,B.melophagi,B.rattimassiliensis,B.rochalimae, B.tribocorum, B.vinsonii, B.washoensis. There were also 27 variants potentially new species. Yunnan,Heilongjiang and Xinjiang have the most abundant Bartonella. Conclusion Bartonella in China is rich in species,and there are many species that can cause human diseases,including some potentially novel species,especially in Yunnan,Heilongjiang,Xinjiang with the highest diversity,which can provide reference for the diagnosis of some diseases of unknown causes.
作者
龙露
陆家海
LONG Lu;LU Jia-hai(School of Public Health,Sun Yat-sen University,Guangzhou,Guangdong 510080,China)
出处
《热带医学杂志》
CAS
2020年第4期455-459,共5页
Journal of Tropical Medicine
基金
国家科技重大专项(2018ZX10101002-001-001)
广东省重点领域研发计划项目(2018B020241002)
广东省科技计划项目(2018B020207013)。
