摘要
在开展三黄连散对人工感染罗非鱼无乳链球菌病预防效果研究的基础上,进一步验证以每千克饲料中添加13.2 g的三黄连散为推荐剂量,在生产中大面积应用的临床效果及安全性。设立三黄连散试验组、中药对照组和空白对照组3个组,每组300尾罗非鱼,连续用药7 d后,每组随机取30尾,测定增重率、器官指数、血液生理生化指标和肝脏抗氧化指标。用药后第8天,采用同居感染的方法感染鱼群,于感染后第14天计算保护率。结果表明,给药7 d后,与空白对照组相比,三黄连散试验组罗非鱼的增重率明显提高,器官指数和多项肝脏抗氧化指标均明显升高,血液生理生化指标总体无明显异常,预防无乳链球菌感染的保护率为43.67%。综上提示,三黄连散能够提高罗非鱼机体抵抗力,降低无乳链球菌感染罗非鱼的死亡率。
In this study,the preventative efficacy of Sanhuanglian Powder against artificially induced Streptococcus agalactiae infection in tilapia was assessed,and the large-scale clinical application effect and safety of Sanhuanglian Powder with recommended dosage of 13.2 g/kg feed in production was evaluated.A total of 3 groups including Sanhuanglian Powder experimental group,traditional Chinese medicine control group,and blank control group were included in this study,and there were 300 tilapia in each group.After a 7-day continuous administration,30 fishes were randomly selected from each group for the measurements of weight gain rate,organ index,blood physiological and biochemical indicators as well as liver antioxidant indexes.On the 8th day after medication,the fishes were challenged with Streptococcus agalactiae by artificial cohabitation infection,and the protection rate was calculated on the 14th day after the challenge.The results showed that after the 7 days administration,compared with the blank control group,Sanhuanglian Powder experimental group had obviously increased weight gain rate,organ index and multiple liver antioxidant indexes,and had no obvious abnormality in blood physiological and biochemical indicators generally.The protective rate of Sanhuanglian Powder against the artificially induced Streptococcus agalactiae infection in tilapia was 43.67%.In conclusion,Sanhuanglian Powder can improve the immune resistance and reduce the mortality rate of tilapia caused by Streptococcus agalactiae infection.
作者
禹方晓
曹迷霞
梁万文
王春会
王爽
韦英益
胡庭俊
YU Fang-xiao;CAO Mi-xia;LIANG Wan-wen;WANG Chun-hui;WANG Shuang;WEI Ying-yi;HU Ting-jun(College of Animal Science and Technology,Guangxi University,Nanning 530005,China;Guangxi Academy of Fishery Science,Nanning 530022,China)
出处
《畜牧与饲料科学》
2020年第3期86-91,共6页
Animal Husbandry and Feed Science
基金
广西创新驱动发展专项资金课题“罗非鱼链球菌病新型快速诊断技术与三黄连中草药复方制剂研发应用”(桂科AA17204081-1)
国家现代农业产业技术体系广西罗非鱼产业创新团队建设专项资金资助课题“罗非鱼链球菌病三黄连合剂的研制”(nycytxgxcxtd-08-02-02)。
关键词
三黄连散
罗非鱼
无乳链球菌
Sanhuanglian Powder
tilapia
Streptococcus agalactiae
